[¹¹C]choline has a weak UV absorption chromophore, and it is challenging to detect less than 1 μg/mL choline using radio-HPLC-UV. In this study, we established an analytical procedure of [¹¹C]choline using the radio-HPLC coupled with the corona-charged aerosol detector. As a result, we achieved more than 100 GBq/μmol (over 0.1 μg/mL of choline) of molar radioactivity at the end of synthesis, over 95% of radiochemical purity, and more than 0.1 μg/mL of 2-dimethyoaminoethanol as a chemical impurity.

中文翻译：

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使用带电晕充电气溶胶检测器的放射性HPLC同时测量[11C]胆碱注射液中的摩尔放射性，放射性化学纯度和化学杂质。

[ ¹¹ C]胆碱具有弱的紫外线吸收发色团，使用放射HPLC-UV检测不到1μg/ mL的胆碱是一项挑战。在这项研究中，我们建立了[ ¹¹ C]胆碱的分析程序，采用了放射性HPLC和带电晕的气溶胶检测器。结果，我们在合成结束时获得了超过100 GBq /μmol（超过0.1μg/ mL的胆碱）的摩尔放射性，超过95％的放射化学纯度，以及超过0.1μg/ mL的2-二甲基氨基乙醇。化学杂质。