Isolation and fast detection of Clostridium perfringens is essential in veterinary medical diagnostics and veterinary research, as it allows to recommend suitable treatment options after antimicrobial resistance determination, and is essential to study pathogenesis. In this study four selective media were tested for the enumeration of, and selectivity towards C. perfringens in faecal samples from poultry. The routinely used Columbia agar with 5% sheep (CBA), Shahidi-Ferguson-perfringens agar (SFP), tryptose sulphite cycloserine agar (TSC), and a novel chromogenic medium, CHROMagar™ C. perfringens (CHCP), were tested. Overall, no difference in C. perfringens recovery could be observed between the selective media. The limit of quantification was 10³ CFU/mL for all agars. CHCP showed the highest specificity, especially when low C. perfringens loads were present in the faeces, with TSC being the second most specific selective medium. Both CBA and SFP allowed considerable growth of other faecal microbiota and were not specific for C. perfringens. On CHCP, differentiation of C. perfringens from other faecal bacteria was straightforward due to the appearance of C. perfringens as orange colonies, with other bacteria being absent or appearing as blue/green colonies. On TSC, C. perfringens appeared as black colonies, but longer incubation periods were sometimes needed for the black colour to develop. Therefore, CHCP can be recommended when timely and easy identification and enumeration of C. perfringens from complex samples, such as faeces, is needed.

产气荚膜梭状芽胞杆菌的分离和快速检测在兽医医学诊断和兽医研究中至关重要，因为它可以在确定抗菌素耐药性后建议合适的治疗方案，并且对发病机理的研究至关重要。在这项研究中，测试了四种选择性培养基对家禽粪便样品中产气荚膜梭菌的计数和选择性。测试了常规使用的含5％羊（CBA）的哥伦比亚琼脂，Shahidi-Ferguson-perfringens琼脂（SFP），亚硫酸胰蛋白丝氨酸环丝氨酸琼脂（TSC）和新型生色培养基CHROMagar™产气荚膜梭状芽胞杆菌（CHCP）。总体而言，选择性培养基之间的产气荚膜梭菌回收率没有差异。定量限为10 所有琼脂为³ CFU / mL。CHCP表现出最高的特异性，尤其是当粪便中产气荚膜梭菌含量低时，TSC是第二高特异性的选择培养基。CBA和SFP都允许其他粪便菌群大量生长，并且对产气荚膜梭菌没有特异性。在CHCP上，由于产气荚膜梭菌显示为橙色菌落，而没有其他细菌或以蓝/绿菌落出现，因此产气荚膜梭菌与其他粪便细菌的区别很简单。在TSC上，产气荚膜梭菌表现为黑色菌落，但有时需要更长的孵育时间才能形成黑色。因此，当需要对粪便等复杂样品中的产气荚膜梭菌进行及时，简便的鉴定和计数时，建议使用CHCP 。