Abstract This study evaluated adhesion and biofilm formation by Candida albicans, Pseudomonas aeruginosa and Staphylococcus epidermidis on surfaces of titanium (Ti) and titanium coated with F18 Bioactive Glass (BGF18). Biofilms were grown and the areas coated with biofilm were determined after 2, 4 and 8 h. Microscopy techniques were applied in order to visualize the structure of the mature biofilm and the extracellular matrix. On the BGF18 specimens, there was less biofilm formation by C. albicans and S. epidermidis after incubation for 8 h. For P. aeruginosa biofilm, a reduction was observed after incubation for 4 h, and it remained reduced after 8 h on BGF18 specimens. All biofilm matrices seemed to be thicker on BGF18 surface than on titanium surfaces. BGF18 showed significant anti-biofilm activity in comparison with Ti in the initial periods of biofilm formation; however, there was extensive biofilm after incubation for 48 h.

中文翻译：

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骨科生物材料：钛植入物上新型生物活性玻璃涂层的抗生物膜活性

摘要 本研究评估了白色念珠菌、铜绿假单胞菌和表皮葡萄球菌对钛 (Ti) 和涂有 F18 生物活性玻璃 (BGF18) 的钛表面的粘附和生物膜形成。生物膜生长，并在 2、4 和 8 小时后测定生物膜覆盖的区域。应用显微镜技术以可视化成熟生物膜和细胞外基质的结构。在 BGF18 标本上，培养 8 小时后，白色念珠菌和表皮葡萄球菌的生物膜形成较少。对于铜绿假单胞菌生物膜，孵育 4 小时后观察到减少，并且在 BGF18 标本上孵育 8 小时后仍保持减少。BGF18 表面上的所有生物膜基质似乎都比钛表面上的更厚。在生物膜形成的初期，BGF18 与 Ti 相比显示出显着的抗生物膜活性；然而，孵育 48 小时后有广泛的生物膜。