Abstract Microbial biofilms are associated with persistent infections because of their high tolerance to antimicrobial agents and host defenses. The effects of centipede oil from Scolopendra subspinipes mutilans and its main components were investigated to identify non-toxic biofilm inhibitors. Centipede oil and linoleic acid at 20 µg ml−1 markedly inhibited biofilm formation by two fluconazole-resistant Candida albicans strains and three Staphylococcus aureus strains without affecting their planktonic cell growth. Also, both centipede oil and linoleic acid inhibited hyphal growth and cell aggregation by C. albicans. In addition, centipede oil and linoleic acid showed anti-biofilm activities against mixed C. albicans and S. aureus biofilms. Transcriptomic analysis showed that centipede oil and linoleic acid downregulated the expressions of several hypha/biofilm-related genes in C. albicans and α-hemolysin in S. aureus. Furthermore, both compounds effectively reduced C. albicans virulence in a nematode infection model with minimal toxicity.

中文翻译：

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蜈蚣油和亚油酸对白色念珠菌和金黄色葡萄球菌生物膜的抑制作用

摘要 微生物生物膜与持续感染有关，因为它们对抗菌剂和宿主防御具有高度的耐受性。研究了来自 Scolopendra subspines muutilans 的蜈蚣油及其主要成分的作用，以确定无毒生物膜抑制剂。20 µg ml-1 的蜈蚣油和亚油酸显着抑制了两种耐氟康唑白色念珠菌菌株和三种金黄色葡萄球菌菌株的生物膜形成，而不影响它们的浮游细胞生长。此外，蜈蚣油和亚油酸均抑制白色念珠菌的菌丝生长和细胞聚集。此外，蜈蚣油和亚油酸对混合的白色念珠菌和金黄色葡萄球菌生物膜显示出抗生物膜活性。转录组学分析表明，蜈蚣油和亚油酸下调白色念珠菌中几种菌丝/生物膜相关基因和金黄色葡萄球菌中α-溶血素的表达。此外，两种化合物都有效地降低了线虫感染模型中白色念珠菌的毒力，且毒性最小。