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Antioxidant, Cytotoxic and Antimicrobial Activity of Chitosan Preparations Extracted from Ganoderma Lucidum Mushroom
Chemistry & Biodiversity ( IF 2.3 ) Pub Date : 2020-06-05 , DOI: 10.1002/cbdv.202000175 Simona Savin 1 , Oana Craciunescu 1 , Anca Oancea 1 , Daniela Ilie 1 , Teodora Ciucan 1 , Loredana Stefania Antohi 1 , Agnes Toma 1 , Alina Nicolescu 2 , Calin Deleanu 2 , Florin Oancea 3
Chemistry & Biodiversity ( IF 2.3 ) Pub Date : 2020-06-05 , DOI: 10.1002/cbdv.202000175 Simona Savin 1 , Oana Craciunescu 1 , Anca Oancea 1 , Daniela Ilie 1 , Teodora Ciucan 1 , Loredana Stefania Antohi 1 , Agnes Toma 1 , Alina Nicolescu 2 , Calin Deleanu 2 , Florin Oancea 3
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Two chitosan extracts were prepared by chemical and enzymatic treatment of Ganoderma lucidum mushroom, as an alternative source to crustacean shells. The molecular weight of the enzymatic extract was lower than that of the chemical one and of shrimp chitosan, as determined by viscosity measurements. Characteristic signals were identified in the 1H‐NMR spectra and high deacetylation degree indicated good physico‐chemical properties for both mushroom chitosan extracts. The scavenging capacity of mushroom chitosan extracts was moderate against the synthetic radicals of 2,2′‐azinobis(3‐ethylbenzothiazoline‐6‐sulfonic acid) (ABTS) and 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH), but higher values were observed for the enzymatic extract, compared to the chemical extract and shrimp chitosan. In vitro cytotoxicity was evaluated in L929 mouse fibroblast cell lines and the results of MTT assay showed good cytocompatibility in the tested range of concentrations. The growth of Gram‐positive bacteria was inhibited more than Gram‐negative bacteria in the presence of mushroom chitosan extracts, in particular by the chemical one, indicating their efficiency as antimicrobial agents. All these results strengthen the evidence of mushroom polysaccharide preparations availability for biomedical applications.
中文翻译：
灵芝提取物壳聚糖制剂的抗氧化、细胞毒和抗菌活性
两种壳聚糖提取物是通过化学和酶处理灵芝蘑菇制备的，作为甲壳类动物壳的替代来源。根据粘度测量结果，酶提取物的分子量低于化学提取物和虾壳聚糖的分子量。在 1H-NMR 光谱中鉴定出特征信号，高脱乙酰度表明两种蘑菇壳聚糖提取物具有良好的理化性质。蘑菇壳聚糖提取物对合成自由基 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) 和 1,1-diphenyl-2-picrylhydrazyl (DPPH) 的清除能力中等，但值较高与化学提取物和虾壳聚糖相比，对酶提取物进行了观察。在 L929 小鼠成纤维细胞系中评估了体外细胞毒性，MTT 测定结果显示在测试浓度范围内具有良好的细胞相容性。在蘑菇壳聚糖提取物的存在下，革兰氏阳性菌的生长比革兰氏阴性菌受到的抑制更多，特别是化学物质，表明它们作为抗菌剂的有效性。所有这些结果加强了蘑菇多糖制剂可用于生物医学应用的证据。
更新日期：2020-06-05
中文翻译：
灵芝提取物壳聚糖制剂的抗氧化、细胞毒和抗菌活性
两种壳聚糖提取物是通过化学和酶处理灵芝蘑菇制备的，作为甲壳类动物壳的替代来源。根据粘度测量结果，酶提取物的分子量低于化学提取物和虾壳聚糖的分子量。在 1H-NMR 光谱中鉴定出特征信号，高脱乙酰度表明两种蘑菇壳聚糖提取物具有良好的理化性质。蘑菇壳聚糖提取物对合成自由基 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) 和 1,1-diphenyl-2-picrylhydrazyl (DPPH) 的清除能力中等，但值较高与化学提取物和虾壳聚糖相比，对酶提取物进行了观察。在 L929 小鼠成纤维细胞系中评估了体外细胞毒性，MTT 测定结果显示在测试浓度范围内具有良好的细胞相容性。在蘑菇壳聚糖提取物的存在下，革兰氏阳性菌的生长比革兰氏阴性菌受到的抑制更多，特别是化学物质，表明它们作为抗菌剂的有效性。所有这些结果加强了蘑菇多糖制剂可用于生物医学应用的证据。
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