Rhizoma Dioscoreae Makino (RDM) is effective in treating gouty arthritis (GA) and hyperuricacidemia, especially in promoting uric acid excretion and reducing the inflammatory reaction. Bioactive constituents in RDM are mainly steroidal saponins such as dioscin, trillin, protodioscin and protogracillin. However, the mechanism of its anti‐GA action is still unclear, owing to the complex pathological and physiological characteristics of GA, and integration of RDM with multiple components, multiple targets and multiple pathways. Herein, a GA rat model was induced with monosodium urate (MSU), and RDM reduced inflammation of rat synovium tissue. Through metabolomics analysis, 35 potential biomarkers with significant changes involved in the pathogenesis of GA induced by MSU were identified, and perturbations were restored after RDM treatment. The most correlated pathways involved in d ‐galactose, d ‐mannose, d ‐glucose, myoinositol, Phosphatidylcholine (PC) (16:0/16:0), LysoPC (15:0), phosphatidic acid (PA) [18:1(9Z )/18:1(11Z )] and glutathione induced by MSU were galactose metabolism, inositol phosphate metabolism, glycerophospholipid metabolism and glutathione metabolism, and the derivations of all those biomarkers could be regulated by RDM treatment. RDM has a therapeutic effect on GA by intervening in changes in endogenous metabolisms and the related metabolic pathways.

中文翻译：

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用UPLC-Q / TOF-MS分析薯Mak总皂苷对痛风性关节炎大鼠的高通量血清代谢组学分析。

薯Di药（RDM）可有效治疗痛风性关节炎（GA）和高尿酸血症，特别是促进尿酸排泄和减少炎症反应。RDM中的生物活性成分主要是甾体皂苷，如薯os皂苷，甘油三酸酯，原薯s皂苷和原皂苷。然而，由于GA复杂的病理和生理特性以及RDM与多种成分，多种靶标和多种途径的整合，其抗GA作用的机制仍不清楚。在本文中，用尿酸钠（MSU）诱导了GA大鼠模型，RDM减轻了大鼠滑膜组织的炎症。通过代谢组学分析，鉴定了35种潜在的生物标志物，这些标志物与MSU诱导的GA的发病机理有关，并且在RDM治疗后恢复了摄动。d-半乳糖，d-甘露糖，d-葡萄糖，肌醇，磷脂酰胆碱（PC）（16：0/16：0），LysoPC（15：0），磷脂酸（PA）[18：1（9 Z）/ 18 ：1（11 Z）]和MSU诱导的谷胱甘肽是半乳糖代谢，肌醇磷酸代谢，甘油磷脂代谢和谷胱甘肽代谢，所有这些生物标记的衍生都可以通过RDM处理来调节。RDM通过干预内源性代谢和相关代谢途径的变化而对GA产生治疗作用。