A reliable and sensitive UPLC–MS/MS method was first established and validated for the simultaneous determination of seven active ingredients of Yaobitong capsule in rat plasma: ginsenoside Rg1, ginsenoside Rb1, osthole, tetrahydropalmatine, paeoniflorin, albiflorin, and ferulic acid. And this method was further applied for the integrated pharmacokinetic study of Yaobitong capsule in rats after oral administration. Plasma samples (100 μL) were precipitated with 300 μL of methanol using carbamazepine as internal standard. Chromatographic separation was achieved using an Aquity UPLC BEH C18 column (100 × 2.1 mm, 1.7 μm), with the mobile phase consisting of 0.1% formic acid and acetonitrile. The method was validated using a good linear relationship (r  ≥ 0.991), and the lower limit of quantification of the analytes ranged from 0.5 to 40 ng/mL. In the integrated pharmacokinetic study, the weight coefficient was calculated by the ratio of AUC_0–∞ of each component to the total AUC_0–∞ of the seven active ingredients. The integrated pharmacokinetic parameters C _max, T _max, and t _1/2 were 81.54 ± 9.62 ng/mL, 1.00 ± 0.21 h, and 3.26 ± 1.14 h, respectively. The integration of pharmacokinetic parameters showed a shorter t _1/2 because of fully considering the contribution of the characteristics of each active ingredient to the overall pharmacokinetics.

中文翻译：

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UPLC-MS / MS同时测定大鼠血浆中腰痹通胶囊中7种有效成分的含量及其在药代动力学中的应用。

首先建立了可靠，灵敏的UPLC-MS / MS方法，并同时测定了大鼠血浆中人参皂苷Rg1，人参皂苷Rb1，osthole，四氢巴马汀，pa药苷，白花青素和阿魏酸的七种有效成分。并将该方法进一步应用于腰痹通胶囊口服给药后大鼠的综合药代动力学研究。使用卡马西平作为内标，用300μL甲醇沉淀血浆样品（100μL）。使用Aquity UPLC BEH C18色谱柱（100×2.1 mm，1.7μm）进行色谱分离，流动相由0.1％甲酸和乙腈组成。该方法已使用良好的线性关系进行了验证（r ≥0.991），分析物的定量下限为0.5至40 ng / mL。在综合药代动力学研究，权重系数被的AUC的比率来计算_0-∞各成分的总AUC _0-∞的七个活性成分。积分药代动力学参数C _max，T _max和t _1/2分别为81.54±9.62 ng / mL，1.00±0.21 h和3.26±1.14 h。由于充分考虑了每种活性成分的特性对整体药代动力学的贡献，因此药代动力学参数的积分显示出较短的t _1/2。