This is the first evidence that replicating vectors can be successfully used for transient protein expression in BY-2 plant cell packs. Transient recombinant protein expression in plants and recently also plant cell cultures are of increasing interest due to the speed, safety and scalability of the process. Currently, studies are focussing on the design of plant virus-derived vectors to achieve higher amounts of transiently expressed proteins in these systems. Here we designed and tested replicating single and multi-cassette vectors that combine elements for enhanced replication and hypertranslation, and assessed their ability to express and particularly co-express proteins by Agrobacterium-mediated transient expression in tobacco BY-2 plant cell packs. Substantial yields of green and red fluorescent proteins of up to ~ 700 ng/g fresh mass were detected in the plant cells along with position-dependent expression. This is the first evidence of the ability of replicating vectors to transiently express proteins in BY-2 plant cell packs.

中文翻译：

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使用单盒和多盒复制载体在烟草 BY-2 植物细胞堆中瞬时表达蛋白质。


这是复制载体可成功用于 BY-2 植物细胞堆中瞬时蛋白表达的第一个证据。由于该过程的速度、安全性和可扩展性，植物中以及最近植物细胞培养物中的瞬时重组蛋白表达越来越受到人们的关注。目前，研究的重点是植物病毒衍生载体的设计，以在这些系统中获得更多的瞬时表达蛋白质。在这里，我们设计并测试了复制单盒和多盒载体，这些载体结合了增强复制和超翻译的元件，并评估了它们在烟草 BY-2 植物细胞包中通过农杆菌介导的瞬时表达表达尤其是共表达蛋白质的能力。在植物细胞中检测到绿色和红色荧光蛋白的大量产量高达约 700 ng/g 新鲜质量以及位置依赖性表达。这是复制载体能够在 BY-2 植物细胞包中瞬时表达蛋白质的能力的第一个证据。