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Mechanisms of interaction of Cetylpyridinium chloride with Staphylococcus aureus in the presence of β-cyclodextrin
Journal of Inclusion Phenomena and Macrocyclic Chemistry ( IF 1.7 ) Pub Date : 2020-04-25 , DOI: 10.1007/s10847-020-00996-x
Thiago M. Miranda , Alan R. Oliveira , Larissa M. D. Andrade , Guilherme F. Silva , Jeferson G. da Silva , Gabriella F. Ferreira , Ângelo M. L. Denadai

Cetylpyridinium chloride (CPC) is a cationic surfactant, which has a biocidal activity against a broad spectrum of bacteria, including Staphylococcus aureus. This microorganism, although frequently found in the normal human microbiota, can become a pathogen that causes a wide variety of infections. Thus, the present work aims to investigate the effect of the β-cyclodextrin (βCD) on CPC antimicrobial activity against S. aureus, especially in the mechanism of interaction with S. aureus membrane. For these purposes, in vitro antimicrobial susceptibility of CPC and CPC/βCD compounds against S. aureus were determined by calculating the lower concentration capable of reducing cell viability by 50 and 100 percent, and the kinetics of bacterial death were evaluated by kill curves for the two systems. After that, the colloidal mechanisms of interaction of the CPC and CPC/βCD against S. aureus were investigated by Dynamic Light Scattering (DLS) and Zeta Potential (ZP) titrations. Finally, the thermodynamic parameters of drug-cell interaction were determined by Isothermal Titration Calorimetry (ITC), in order to obtain deeper understanding of the mechanism of drug interactions. The results of DLS, ZP and ITC showed that in presence of βCD occurs a different mechanism of interaction with S. aureus membrane, explaining therefore the higher antimicrobial activity of CPC/βCD system.

中文翻译:

β-环糊精存在下氯化十六烷基吡啶与金黄色葡萄球菌相互作用的机制

氯化十六烷基吡啶 (CPC) 是一种阳离子表面活性剂,对包括金黄色葡萄球菌在内的广谱细菌具有杀生物活性。这种微生物虽然常见于正常人类微生物群中,但可以成为引起多种感染的病原体。因此,目前的工作旨在研究 β-环糊精 (βCD) 对 CPC 对金黄色葡萄球菌的抗菌活性的影响,特别是在与金黄色葡萄球菌膜相互作用的机制中。出于这些目的,CPC 和 CPC/βCD 化合物对金黄色葡萄球菌的体外抗微生物敏感性通过计算能够将细胞活力降低 50% 和 100% 的较低浓度来确定,并通过杀灭曲线评估细菌死亡的动力学两个系统。之后,通过动态光散射 (DLS) 和 Zeta 电位 (ZP) 滴定法研究了 CPC 和 CPC/βCD 与金黄色葡萄球菌相互作用的胶体机制。最后,通过等温滴定量热法(ITC)测定药物-细胞相互作用的热力学参数,以期更深入地了解药物相互作用的机理。DLS、ZP 和 ITC 的结果表明,在 βCD 存在下,与金黄色葡萄球菌膜发生不同的相互作用机制,因此解释了 CPC/βCD 系统具有更高的抗菌活性。以便更深入地了解药物相互作用的机制。DLS、ZP 和 ITC 的结果表明,在 βCD 存在下,与金黄色葡萄球菌膜发生不同的相互作用机制,因此解释了 CPC/βCD 系统具有更高的抗菌活性。以便更深入地了解药物相互作用的机制。DLS、ZP 和 ITC 的结果表明,在 βCD 存在下,与金黄色葡萄球菌膜发生不同的相互作用机制,因此解释了 CPC/βCD 系统具有更高的抗菌活性。
更新日期:2020-04-25
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