Microfluidic paper-based analytical devices (µPADs) take the paper as a base material and integrate nanoscale microchannel on it for multiple detections. Its unique properties like low cost, portability, simple operation, and easy to save make it better than the traditional microfluidic chips. While designed originally for point-of-care medical diagnostics, µPADs have attracted the attention of many researchers in the fields of environmental monitoring, water quality, and food safety. The novelty of this paper is to present a detailed overview of µPADs for clinical applications. Firstly, a brief introduction to production methods, characteristics, and applications of these methods have been given. Secondly, the basic implementation, working principles, and corresponding performance of detection methods of clinical devices have been discussed, which enable the µPADs to detect biomarkers, human cells, bacteria, and viruses in a short time. Lastly, the factors that limit µPADs commercial applications, and their future research directions have also been briefly summarized.

中文翻译：

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临床应用中的微流体纸基分析装置

微流体纸基分析装置 (μPAD) 以纸为基材，在其上集成纳米级微通道以进行多次检测。其低成本、便携、操作简单、易于保存等独特性能使其优于传统的微流控芯片。虽然最初设计用于即时医疗诊断，但 µPAD 已引起环境监测、水质和食品安全领域许多研究人员的关注。本文的新颖之处在于详细概述了用于临床应用的 µPAD。首先简要介绍了这些方法的生产方法、特点和应用。其次，讨论了临床器械检测方法的基本实现、工作原理和相应性能，这使 µPAD 能够在短时间内检测生物标志物、人体细胞、细菌和病毒。最后，还简要总结了限制μPADs商业应用的因素及其未来的研究方向。