Mast cells play a significant role in urticaria pathogenesis. It's evidenced that vitamin D has positive impact in chronic spontaneous urticaria (CSU) recently, but underlying mechanisms remain unclear. In this study, isobaric tag for relative and absolute quantification-liquid chromatography-mass spectrometer/mass spectrometer was used to detect the expression of proteins in sera of CSU patients and healthy subjects. Thirty-one differentially expressed proteins were identified, in which vitamin D binding protein (VDBP) was higher in CSU patients than that in healthy subjects after verification. Our results indicated that sera of CSU patients induced the production of vascular endothelial growth factor (VEGF) in mast cells through PI3K/Akt/p38 MAPK/HIF-1α axis in an IgE-depended way, and 25(OH)D3 suppressed the expression of VEGF by inhibiting this signaling pathway axis in this process. Collectively, these results suggest VDBP to be a potential biomarker and propose a potential mechanism of benefit for vitamin D therapy in CSU.

中文翻译：

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维生素D通过抑制慢性自发性荨麻疹中的PI3K / Akt / p38 MAPK /HIF-1α途径来抑制肥大细胞中血管内皮生长因子的产生。

肥大细胞在荨麻疹发病机理中起重要作用。有证据表明，维生素D最近在慢性自发性荨麻疹（CSU）中具有积极作用，但其潜在机制尚不清楚。在这项研究中，使用了相对定量和绝对定量的等压标记-液相色谱-质谱仪/质谱仪检测CSU患者和健康受试者血清中的蛋白质表达。经鉴定，鉴定出三十一种差异表达蛋白，其中CSU患者的维生素D结合蛋白（VDBP）高于健康受试者。我们的结果表明，CSU患者的血清通过PI3K / Akt / p38 MAPK /HIF-1α轴以依赖IgE的方式诱导了肥大细胞中血管内皮生长因子（VEGF）的产生，25（OH）D3在此过程中通过抑制该信号通路轴来抑制VEGF的表达。总体而言，这些结果表明VDBP是潜在的生物标志物，并提出了对CSU中维生素D治疗有益的潜在机制。