At the subcellular level, the cytoskeleton regulates cell structure, organelle movement, and cytoplasmic streaming. Autophagy is a process to remove unwanted biomaterials or damaged organelles through double membrane compartments known as autophagosomes. Autophagosome biogenesis requires vesicle trafficking between donor and acceptor compartments, membrane expansion, and fusion, which is very likely to be regulated by the cytoskeleton. Recent studies have demonstrated that by knocking out key actin-regulating proteins, autophagosome biogenesis is inhibited. However, the formation of ATG8 positive structures are not affected when the entire actin network is disrupted. Here, we discuss this paradox and propose the function of the actin cytoskeleton in plant autophagy.

在亚细胞水平上，细胞骨架调节细胞结构，细胞器运动和细胞质流。自噬是通过称为自噬体的双膜隔室去除不需要的生物材料或受损细胞器的过程。自噬体的生物发生需要供体和受体区室之间的囊泡运输，膜膨胀和融合，这很可能受细胞骨架的调节。最近的研究表明，通过敲除关键的肌动蛋白调节蛋白，自噬体的生物发生受到抑制。但是，当整个肌动蛋白网络被破坏时，ATG8阳性结构的形成不会受到影响。在这里，我们讨论这个悖论，并提出肌动蛋白细胞骨架在植物自噬中的功能。