A novel glucose biosensor was fabricated by loading a glassy carbon electrode with a 1-methyl imidazole based ionic liquid-functionalized graphene/carbon nanotubes(CNTs) composite, which was used to immobilize horse radish peroxidase (HRP) and glucose oxidase (GOD) for glucose detection. SEM, AFM and FTIR were used to investigate the microstructure and morphology of the electrode. The electrochemical performance of the electrode was investigated by cyclic voltammetry(CV) and electrochemical impedance spectroscopy. Results indicates that the electrode has excellent electrocatalytic activity towards glucose. The biosensor prepared at the optimal loading amount and ratio of HRP to GOD has a linear range of 0.004-5 mmol/L, a detection limit of 3.99×10⁻⁷mol/L and a sensitivity of 53.89 μA mmol/L⁻¹cm⁻² for glucose detection with an excellent stability and reproducibility. The excellent performance of the biosensor is ascribed to the stable three-dimensional structure, where graphene sheets provide a large surface area for immobilizing two enzymes and CNTs insert between graphene sheets to decrease electron transfer resistance.

通过在玻璃碳电极上负载1-甲基咪唑基离子液体功能化石墨烯/碳纳米管（CNTs）复合材料制备了一种新型葡萄糖生物传感器，该化合物用于固定辣根过氧化物酶（HRP）和葡萄糖氧化酶（GOD）的活性。葡萄糖检测。SEM，AFM和FTIR用于研究电极的微观结构和形态。通过循环伏安法和电化学阻抗谱研究了电极的电化学性能。结果表明该电极对葡萄糖具有优异的电催化活性。以最佳的HRP与GOD的负载量和比例制备的生物传感器的线性范围为0.004-5 mmol / L，检测极限为3.99×10 ^-7 mol / L，灵敏度为53.89μAmmol / L ^-1cm ^-2用于葡萄糖检测，具有出色的稳定性和可重复性。生物传感器的出色性能归因于稳定的三维结构，其中石墨烯片提供了较大的表面积来固定两种酶，而CNT插入石墨烯片之间以降低电子传递阻力。