A highly cholesterol-diet is associated with atherosclerosis and little about the development of cerebellar cortex disorder. The study illustrated the changes of cerebellar cortex of rat neonate maternally fed on high cholesterol diet and the capacity of pomegranate alone or in combination with atorvastatin to improve it. Eighty-eight pregnant Wister rats were divided into eight groups (n = 11); control, pomegranate supplemented group (daily orally 0.4 ml (20%), atorvastatin (10 mg/kg BT), hypercholesterolemia (dietary consumption 3% cholesterol for 6 weeks prior to conception and throughout gestation and lactation period), hypercholesterolemia and pomegranate or atorvastatin, hypercholesterolemia and atorvastatin and pomegranate. Dams and their offspring were sacrificed at 21 days post-partum. Sera of mother and cerebellum of offspring were investigated biochemically as well as histo-cytological changes of cerebellar cortex of offspring. Offspring maternally fed on high cholesterol diet showed damage of the cerebellar Purkinje and granular cells associated with demyelination, increased caspase 3 immunohistochemistry and increased DNA damage. These were associated with decreased brain neurotransmitters and increase apoptic markers. Dams supplemented pomegranate and/or atorvastatin improved the assayed parameters more than that of atorvastatin alone. The authors concluded that pomegranate juice contains potent antioxidant nutrients capable of reducing the cytotoxicity of hypercholesterolemia and atorvastatin, and enhancing the structure and function of the cerebellar.

中文翻译：

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石榴汁和阿托伐他汀对改善高胆固醇饮食母乳喂养大鼠小脑结构和功能的协同作用

高胆固醇饮食与动脉粥样硬化有关，而与小脑皮质疾病的发展关系不大。该研究说明了母体喂养高胆固醇饮食的大鼠新生儿小脑皮层的变化，以及石榴单独或与阿托伐他汀联合使用的改善能力。88 只怀孕的 Wister 大鼠被分成 8 组（n = 11）；对照组，石榴补充组（每日口服 0.4 毫升（20%），阿托伐他汀（10 毫克/千克 BT），高胆固醇血症（受孕前 6 周以及整个妊娠和哺乳期饮食消耗 3% 胆固醇），高胆固醇血症和石榴或阿托伐他汀、高胆固醇血症和阿托伐他汀和石榴。在产后 21 天处死母畜及其后代。对母亲和后代小脑的血清进行生化研究以及后代小脑皮质的组织细胞学变化。以高胆固醇饮食为母体喂养的后代表现出与脱髓鞘相关的小脑浦肯野和颗粒细胞损伤、半胱天冬酶 3 免疫组织化学增加和 DNA 损伤增加。这些与脑神经递质减少和凋亡标记物增加有关。Dams 补充石榴和/或阿托伐他汀比单独使用阿托伐他汀更能改善测定参数。作者得出结论，石榴汁含有有效的抗氧化营养素，能够降低高胆固醇血症和阿托伐他汀的细胞毒性，并增强小脑的结构和功能。