Abstract Mutant crtRB1 gene has contributed to provitamin-A (proA) rich maize cultivars worldwide. However, considerable degradation of proA during storage reduces its efficacy. Here, 22 biofortified inbreds with crtRB1 were stored for 6-months. Refrigerated and vacuum storage were significantly better than traditional storage for retention of both proA and non-proA carotenoids. Under traditional storage, β-cryptoxanthin (20.7%) had higher retention than β-carotene (16.3%), while lutein (53.9%) had better retention over zeaxanthin (42.4%) among crtRB1-inbreds. Major fraction of proA (68.8%) was lost during first three months of storage, while non-proA showed slow and progressive degradation (3-months: 28.5% loss, 6-months: 49.4% loss) under traditional storage. HKI161PV (27.4%), HP465-41 (26.9%), HKI193–1 PV (25.9%) and HP465-30 (25.7%) were the most potential inbreds with >25% retention of proA compared to 8–10% retention among crtRB1-based inbreds. Promising inbreds can be used as donor in biofortification programme, while contrasting inbreds distinctly differing for retention of proA carotenoids can be used to map loci for higher retention of proA.

中文翻译：

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不同贮藏条件下亚热带生物强化玉米籽粒类胡萝卜素保留的遗传变异性分析

摘要 crtRB1 突变基因对世界范围内富含维生素原 A (proA) 的玉米品种做出了贡献。然而，proA 在储存过程中的显着降解会降低其功效。在这里，22 只具有 crtRB1 的生物强化近交被储存了 6 个月。冷藏和真空储存在 proA 和非 proA 类胡萝卜素的保留方面明显优于传统储存。在传统储存下，β-隐黄质 (20.7%) 的保留率高于 β-胡萝卜素 (16.3%)，而叶黄素 (53.9%) 在 crtRB1 近交系中的保留率高于玉米黄质 (42.4%)。proA 的主要部分 (68.8%) 在存储的前三个月丢失，而非 proA 在传统存储下表现出缓慢和渐进的降解（3 个月：28.5% 损失，6 个月：49.4% 损失）。HKI161PV (27.4%)、HP465-41 (26.9%)、HKI193-1 PV (25.9%) 和 HP465-30 (25. 7%) 是最具潜力的近交系，proA 的保留率大于 25%，而基于 crtRB1 的近交系的保留率为 8-10%。有前途的近交系可用作生物强化计划的供体，而在 proA 类胡萝卜素保留方面明显不同的对比近交系可用于绘制基因座以提高 proA 的保留率。