Abstract Osteoarthritis (OA) is a degenerative joint disease, which is usually accompanied by inflammation. The aim of this study was to evaluate the anti-inflammatory effect of Low-intensity pulsed ultrasound (LIPUS) on OA. Fibroblast-Like Synoviocyte (FLS) were stimulated by LIPUS with/without interleukin (IL)-1β to evaluate inflammatory responses. The results showed that IL-1β could effectively induce the production of nitric oxide (NO), prostaglandin E2 (PGE2), matrix metalloprotease (MMP)-1, MMP-3 in FLS. LIPUS could effectively inhibit the induction of NO, PGE2, MMP-1 and MMP-3. In addition, the activation of the NF-κB pathway induced by IL-1β was diminished by LIPUS treatment. We found that the LIPUS alleviated IL-1β-induced inflammatory responses and catabolic effect in FLS via regulating the NF-κB pathway, and ultrasound intensity and treatment time should be optimized. Thus, LIPUS can be used as a new therapeutic agent for the treatment of OA.

中文翻译：

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低强度脉冲超声通过 NF-κB 通路抑制 IL-1β 诱导的成纤维细胞样滑膜细胞炎症

摘要 骨关节炎（OA）是一种退行性关节疾病，通常伴有炎症。本研究的目的是评估低强度脉冲超声 (LIPUS) 对 OA 的抗炎作用。成纤维细胞样滑膜细胞 (FLS) 由含/不含白介素 (IL)-1β 的 LIPUS 刺激以评估炎症反应。结果表明IL-1β能有效诱导FLS产生一氧化氮(NO)、前列腺素E2(PGE2)、基质金属蛋白酶(MMP)-1、MMP-3。LIPUS能有效抑制NO、PGE2、MMP-1和MMP-3的诱导。此外，LIPUS 治疗减少了 IL-1β 诱导的 NF-κB 通路的激活。我们发现 LIPUS 通过调节 NF-κB 通路减轻了 FLS 中 IL-1β 诱导的炎症反应和分解代谢作用，应优化超声强度和治疗时间。因此，LIPUS 可用作治疗 OA 的新治疗剂。