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A safe and reusable imaging chamber compatible with organic solvents
Microscopy ( IF 1.5 ) Pub Date : 2020-04-25 , DOI: 10.1093/jmicro/dfaa020
Martin Colombini 1 , Pawel Pasierbek 2 , Wouter Masselink 1
Affiliation  

High refractive index organic solvents are commonly used as an imaging medium in tissue clearing approaches. While effective, such solvents provide serious concerns for the safety of users and the equipment, especially in a central microscopy unit. To overcome these concerns, we have developed a large and re-usable imaging chamber compatible with the universal mounting frame AK (PeCon GmbH). This chamber is easy to assemble, and significantly improves the working environment in a central microscopy unit, where hazardous chemicals could negatively affect equipment and people. To encourage the uptake of these chambers, the design is made publicly available for download.

中文翻译:

与有机溶剂兼容的安全且可重复使用的成像室

高折射率有机溶剂通常用作组织清除方法中的成像介质。虽然有效,但此类溶剂会严重影响用户和设备的安全,尤其是在中央显微镜装置中。为了克服这些问题,我们开发了一种与通用安装框架 AK (PeCon GmbH) 兼容的大型且可重复使用的成像室。该室易于组装,并显着改善了中央显微镜装置的工作环境,在那里危险化学品可能会对设备和人员产生负面影响。为了鼓励这些商会的采用,该设计公开可供下载。
更新日期:2020-04-25
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