To investigate genomic pathways that may influence physiology and infectivity during the menstrual cycle, RNA sequence analysis was performed on patient-matched engineered ectocervical tissue after follicular and luteal phase hormone treatments. We developed distinct cellular, molecular and biological profiles in ectocervical epithelium dependent on menstrual cycle phase. Follicular phase hormones were associated with proliferation, transcription, and cell adhesion, while luteal phase samples expressed genes involved in immune cell recruitment, inflammation, and protein modifications. Additionally, our analysis revealed mucins not previously reported in ectocervical tissue, which could play an important role in fertility and disease prevention. This study provides insight into the phenomenon of increased luteal phase vulnerability to infection and identifyies potential targets for future research.

中文翻译：

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依赖于月经周期阶段的工程人类宫颈组织中不同的卵泡和黄体转录谱。

为了研究可能影响月经周期生理学和传染性的基因组途径，在卵泡期和黄体期激素治疗后对患者匹配的工程外宫颈组织进行了 RNA 序列分析。我们在取决于月经周期阶段的宫颈外上皮中开发了不同的细胞、分子和生物学特征。卵泡期激素与增殖、转录和细胞粘附有关，而黄体期样本表达的基因与免疫细胞募集、炎症和蛋白质修饰有关。此外，我们的分析揭示了先前未在宫颈外组织中报道的粘蛋白，这可能在生育和疾病预防中发挥重要作用。