Volatile thiols are not present in must but are synthesized and released by wine yeasts during alcoholic fermentation. In this study, autochthonous and commercial Saccharomyces cerevisiae strains were characterized for the expression of the main genes involved in thiols metabolism and their production in wine. New primer sets were developed on the basis of the S288c genome to evaluate the expression of Cys3, Cys4, MET17 and IRC7 genes. Obtained data revealed the occurrence of some thiols, for example, 4‐mercapto‐4‐methylpentan‐2‐one (4‐MMP) and 3‐mercaptohexan‐1‐ol (3‐MH) in Pecorino white wine. All genes were upregulated, but only for IRC7 was found a correlation with 4‐MMP release: strains with the highest production showed the highest transcription level. IRC7 gene could be proposed as target for the selection of S. cerevisiae strains to increase thiols content in wine.

中文翻译：

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酿酒酵母中 IRC7 基因表达与 4-mercapto-4-methylpentan-2-one 产量的相关性。

挥发性硫醇不存在于葡萄汁中，而是在酒精发酵过程中由葡萄酒酵母合成和释放。在这项研究中，本土和商业酿酒酵母菌株的特征在于参与硫醇代谢及其在葡萄酒中生产的主要基因的表达。在 S288c 基因组的基础上开发了新的引物组，以评估Cys 3、Cys 4、MET 17 和IRC 7 基因的表达。获得的数据揭示了一些硫醇的存在，例如 4-mercapto-4-methylpentan-2-one (4-MMP) 和 3-mercaptohexan-1-ol (3-MH) 在 Pecorino 白葡萄酒中。所有基因都被上调，但仅针对IRC7 被发现与 4-MMP 释放相关：产量最高的菌株表现出最高的转录水平。IRC 7 基因可作为选择酿酒酵母菌株以增加葡萄酒中硫醇含量的靶标。