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Cadmium-induced toxicity increases prolyl endopeptidase (PREP) expression in the rat testis.
Molecular Reproduction and Development ( IF 2.7 ) Pub Date : 2020-04-24 , DOI: 10.1002/mrd.23345 Massimo Venditti 1 , Marouane Chemek 2 , Sergio Minucci 1 , Imed Messaoudi 2
Molecular Reproduction and Development ( IF 2.7 ) Pub Date : 2020-04-24 , DOI: 10.1002/mrd.23345 Massimo Venditti 1 , Marouane Chemek 2 , Sergio Minucci 1 , Imed Messaoudi 2
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During the differentiation of the male gamete, there is a massive remodeling in the shape and architecture of all the cells of the seminiferous epithelium. The cytoskeleton, as well as many associated proteins with it, plays a pivotal role in this process. The testis is particularly susceptible to environmental pollutant, which can lead to injury and impairment of normal spermatozoa production. Cadmium (Cd) is one of the major chemical environmental toxicants in economically developed countries. Food and cigarettes are the main sources of exposure to this element. Here, the protective role of zinc (Zn) to prevent the testicular toxicity in male adult rats after prenatal and during lactation exposure to Cd has been assessed. Altered testicular histology at the interstitial and germinal levels was found, whereas Zn supply completely corrected Cd toxicity. Moreover, the effects of these metals on the testicular expression and localization of the protease prolyl endopeptidase (PREP) were evaluated. Interestingly, the results showed an increase of PREP messenger RNA and protein. Data were corroborated by immunofluorescence. This study raises the possibility of using PREP as a new fertility marker.
中文翻译：
镉诱导的毒性会增加大鼠睾丸中脯氨酰内肽酶（PREP）的表达。
在雄配子分化过程中，生精上皮所有细胞的形状和结构发生了巨大的重塑。细胞骨架以及与其相关的许多蛋白质在此过程中起着关键作用。睾丸特别容易受到环境污染物的影响，这可能导致伤害和正常精子生产的损害。镉（Cd）是经济发达国家的主要化学环境毒物之一。食物和香烟是接触该元素的主要来源。在这里，已经评估了锌（Zn）预防产前和哺乳期Cd暴露对成年雄性大鼠睾丸毒性的保护作用。发现间质和生发水平睾丸组织学改变，而锌的供应完全纠正了镉的毒性。此外，评估了这些金属对睾丸表达和蛋白酶脯氨酰内肽酶（PREP）定位的影响。有趣的是，结果显示PREP信使RNA和蛋白质增加。免疫荧光证实了数据。这项研究提出了将PREP用作新的生育指标的可能性。
更新日期：2020-04-24
中文翻译：
镉诱导的毒性会增加大鼠睾丸中脯氨酰内肽酶（PREP）的表达。
在雄配子分化过程中，生精上皮所有细胞的形状和结构发生了巨大的重塑。细胞骨架以及与其相关的许多蛋白质在此过程中起着关键作用。睾丸特别容易受到环境污染物的影响，这可能导致伤害和正常精子生产的损害。镉（Cd）是经济发达国家的主要化学环境毒物之一。食物和香烟是接触该元素的主要来源。在这里，已经评估了锌（Zn）预防产前和哺乳期Cd暴露对成年雄性大鼠睾丸毒性的保护作用。发现间质和生发水平睾丸组织学改变，而锌的供应完全纠正了镉的毒性。此外，评估了这些金属对睾丸表达和蛋白酶脯氨酰内肽酶（PREP）定位的影响。有趣的是，结果显示PREP信使RNA和蛋白质增加。免疫荧光证实了数据。这项研究提出了将PREP用作新的生育指标的可能性。
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