Nowadays, the main limitation for clinical application of scaffolds is considered to be an insufficient vascularization of the implanted platforms and healing tissues. In our studies, we proposed a novel PLA-based hybrid platform with aligned and random fibrous internal structure and incorporated calcium phosphate (CaP) ormoglass nanoparticles (0, 10, 20 and 30 wt%) as an off-the-shelf method for obtaining scaffolds with pro-angiogenic properties. Complex morphological and physicochemical evaluation of PLA–CaP ormoglass composites was performed before and after in vitro degradation test in SBF solution to assess their biological potential. The degradation process of PLA–CaP ormoglass composites was accompanied by numerous CaP-based precipitations with extended topography and cauliflower-like shape which may enhance bonding of the material with the bone tissue and accelerate the regenerative process. Random fiber orientation was preferable for CaP compounds deposition upon in vitro degradation. CaP compounds precipitated firstly for randomly oriented composite nonwovens with 20 and 30 wt% addition of ormoglass. Moreover, the preliminary bioactivity test has shown that BSA adsorbed to PLA–CaP ormoglass composites (both aligned and randomly oriented) with 20 and 30 wt% of ormoglass nanoparticles which was not observed for pure PLA scaffolds.

中文翻译：

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可降解电纺丝聚乳酸/生物活性磷酸钙或玻璃支架的体外评估

如今，支架临床应用的主要限制被认为是植入平台和愈合组织的血管化不足。在我们的研究中，我们提出了一种新颖的基于PLA的混合平台，该平台具有对齐的且随机的纤维内部结构，并结合了磷酸钙（CaP）或玻璃纳米粒子（0、10、20和30 wt％）作为现成的获得方法具有促血管生成特性的支架。在SBF溶液中进行体外降解测试之前和之后，对PLA–CaP或有机玻璃复合材料进行了复杂的形态和理化评估，以评估其生物潜力。PLA–CaP或有机玻璃复合材料的降解过程伴随着大量基于CaP的沉淀，具有扩展的形貌和菜花状形状，这可能会增强材料与骨组织的结合并加速再生过程。对于CaP化合物在体外降解后的沉积，随机纤维取向是优选的。对于随机取向的复合非织造布，CaP化合物首先沉淀出来，添加了20％和30％的Ormoglass。此外，初步的生物活性测试表明，BSA吸附有20和30 wt％的Ormoglass纳米颗粒的PLA-CaP Ormoglass复合材料（既对齐又随机定向），这在纯PLA支架中没有观察到。对于CaP化合物在体外降解后的沉积，随机纤维取向是优选的。对于随机取向的复合非织造布，CaP化合物首先沉淀出来，添加了20％和30％的Ormoglass。此外，初步的生物活性测试表明，BSA吸附有20和30 wt％的Ormoglass纳米颗粒的PLA-CaP Ormoglass复合材料（既对齐又随机定向），这在纯PLA支架中没有观察到。对于CaP化合物在体外降解后的沉积，随机纤维取向是优选的。对于随机取向的复合非织造布，CaP化合物首先沉淀出来，添加了20％和30％的Ormoglass。此外，初步的生物活性测试表明，BSA吸附有20和30 wt％的Ormoglass纳米颗粒的PLA-CaP Ormoglass复合材料（既对齐又随机定向），这在纯PLA支架中没有观察到。