Alteration of protein homeostasis mediates the interaction of Pseudomonas aeruginosa with Staphylococcus aureus.,Molecular Microbiology

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Alteration of protein homeostasis mediates the interaction of Pseudomonas aeruginosa with Staphylococcus aureus.
Molecular Microbiology ( IF 2.6 ) Pub Date : 2020-04-22 , DOI: 10.1111/mmi.14519
Nana Yang _{1,

2} , Qiao Cao _{2,

3} , Shuyang Hu _{1,

2} , Chenchen Xu _{1,

2} , Ke Fan _{1,

2} , Feifei Chen _{2,

3} , Cai-Guang Yang _{1,

2} , Haihua Liang ₃ , Min Wu ₄ , Taeok Bae ₅ , Lefu Lan _{1,

2,

3,

6}

Affiliation

Intracellular protein degradation is essential for the survival of all organisms, but its role in interspecies interaction is unknown. Here, we show that the ClpXP protease of Pseudomonas aeruginosa suppresses its antimicrobial activity against Staphylococcus aureus, a common pathogen co‐isolated with P. aeruginosa from polymicrobial human infections. Using proteomic, biochemical, and molecular genetic approaches, we found that this effect is due to the inhibitory effects of ClpXP on the quorum sensing (QS) of P. aeruginosa, mainly by degrading proteins (e.g., PhnA, PhnB, PqsR, and RhlI) which are critical for the production of QS signal molecules PQS and C4‐HSL. We provide evidence that co‐culturing with S. aureus induces a decrease in the activity of ClpXP in P. aeruginosa, an effect which was also achieved by the treatment of P. aeruginosa with N‐acetylglucosamine (GlcNAc), a widespread chemical present on the surface of diverse cell types from bacteria to humans. These findings extend the range of biological events governed by proteolytic machinery to microbial community structure, thus also suggesting that a chemical‐induced alteration of protein homeostasis is a mechanism for interspecies interactions.

中文翻译：

蛋白质稳态的改变介导了铜绿假单胞菌与金黄色葡萄球菌的相互作用。

细胞内蛋白质降解对于所有生物的生存都是必不可少的，但其在种间相互作用中的作用尚不清楚。在这里，我们显示铜绿假单胞菌的ClpXP蛋白酶抑制了其对金黄色葡萄球菌的抗菌活性，金黄色葡萄球菌是与铜绿假单胞菌共同分离出的多病菌人类感染的常见病原体。使用蛋白质组学，生化和分子遗传学方法，我们发现这种效果是由于ClpXP对铜绿假单胞菌的群体感应（QS）的抑制作用，主要是通过降解蛋白质（例如PhnA，PnB，PqsR和RhlI）），这对于产生QS信号分子PQS和C4-HSL至关重要。我们提供的证据表明金黄色葡萄球菌引起铜绿假单胞菌ClpXP活性的降低，也可以通过用N-乙酰氨基葡糖（GlcNAc）处理铜绿假单胞菌来实现，N-乙酰氨基葡萄糖（GlcNAc）是细菌中多种细胞类型表面上普遍存在的化学物质对人类。这些发现将由蛋白水解机制控制的生物学事件的范围扩展至微生物群落结构，因此也表明，化学诱导的蛋白质稳态的改变是种间相互作用的机制。

更新日期：2020-04-22

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