A Gram-negative aerobic bacterium, designated RR4-38^T, was isolated from a biofilter in a seawater recirculating aqua-culture system (RAS) in Busan, South Korea. The bacteria were irregular, short, rod-shaped, non-motile, oxidase-positive, and catalase-negative. Growth of the strain RR4-38^T was observed at 15–35·C (optimum, 25–30·C), pH 5.5–9.5 (optimum, pH 8.0), and in the presence of 0–5% (w/v) NaCl (optimum, 3%). Phylogenetic analysis based on the 16S rRNA gene sequences showed that the strain RR4-38^T formed a distinct lineage with close genera Ulvibacter (≤ 95.01% 16S rRNA gene sequence similarity), Aureitalea (94.74%), Aureisphaera (≤ 93.27%), and Jejudonia (93.07%) that all belong to the family Flavobacteriaceae. Whole-genome sequence comparison revealed that the ANI (average nucleotide identity) and digital DDH (DNA-DNA hybridization) values between strain RR4-38^T and the two closest strains, Ulvibacter antarcticus DSM 23424^T and Aureitalea marina S1-66^T, were 68.96–69.88% and 17.4–19%, respectively. The genome analysis revealed that the strain might be involved in biodegradation of organic debris produced by farmed fish in aquaculture systems. The predominant respiratory quinone was menaquinone MK-6 and the major cellular fatty acids were iso-C_15:0 (26.5%), iso-C_17:0 3-OH (16.4%), iso-C_15:1 G (15%), and iso-C_16:0 3-OH (9.6%). The major cellular polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, unidentified aminolipids, and glycolipids. Based on phenotypic, chemotaxonomic, and phylogenetic features, strain RR4-38^t represents a novel genus and species in the family Flavobacteriaceae, for which the name Pukyongia salina gen. nov., sp. nov. is proposed. The type strain is RR4-38^T (= KCTC 52651^T = DSM 108068^T).

中文翻译：

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Pukyongia salina gen. 十一月，sp。十一月，黄杆菌科的一个新属。

从韩国釜山海水再循环水产养殖系统 (RAS) 的生物过滤器中分离出一种革兰氏阴性需氧菌，命名为 RR4-38 ^T。细菌是不规则的、短的、杆状的、不活动的、氧化酶阳性和过氧化氢酶阴性。菌株 RR4-38 ^T的生长在 15–35·C（最佳，25–30·C）、pH 5.5–9.5（最佳，pH 8.0）和存在 0–5%（w/v ) NaCl（最佳，3%）。基于 16S rRNA 基因序列的系统发育分析表明，菌株 RR4-38 ^T与Ulvibacter属（≤ 95.01% 16S rRNA 基因序列相似性）、Aureitalea（94.74%）、Aureisphaera（≤93.27%）和空肠属(93.07%) 均属于黄杆菌科。全基因组序列比较表明，菌株 RR4-38 ^T和两个最接近的菌株南极锈菌DSM 23424 ^T和Aureitalea marina S1-66 ^T之间的 ANI（平均核苷酸同一性）和数字 DDH（DNA-DNA 杂交）值是分别为 68.96–69.88% 和 17.4–19%。基因组分析表明，该菌株可能参与水产养殖系统中养殖鱼类产生的有机碎片的生物降解。主要的呼吸醌是甲基萘醌 MK-6，主要的细胞脂肪酸是 iso-C _15:0 (26.5%)、iso-C _17:03-OH (16.4%)、iso-C _15:1 G (15%) 和iso-C _16:0 3-OH (9.6%)。主要的细胞极性脂质是二磷脂酰甘油、磷脂酰乙醇胺、未鉴定的氨基脂和糖脂。基于表型、化学分类学和系统发育特征，菌株 RR4-38 ^t代表黄杆菌科中的一个新属和新种，因此命名为Pukyongia salina gen。十一月，sp。十一月 被提议。类型菌株是 RR4-38 ^T（= KCTC 52651 ^T = DSM 108068 ^T）。