This study investigated the obstacles in detecting enteric viruses from berry fruits, which are on the one hand often associated with outbreaks of viral enteric disease, and on the other hand recognized as a challenging food matrix for molecular detection of enteric viruses. According to the ISO 15216 protocol, for soft fruit samples, virus extraction is by elution with agitation followed by precipitation with polyethylene glycol/NaCl. As a result, first, the phenolic content in the berry eluate was found to be weakly correlated with the detection of coliphage MS2 spiked in the berry samples. Second and more importantly, it was observed that the gel-like pellets formed after precipitation could entrap considerable portions of viruses from being further purified and recovered for detection, suggesting that the low virus detection sensitivity from berries is largely due to the pectin content with complicated chemical structures in the berry fruits. Future research is needed to solve this problem in a targeted way.

中文翻译：

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发现从浆果中检测肠病毒的障碍物。

这项研究调查了从浆果中检出肠病毒的障碍，这些障碍通常与病毒性肠病的爆发有关，另一方面被认为是对肠病毒进行分子检测的具有挑战性的食物基质。根据ISO 15216协议，对于软水果样品，病毒提取是通过搅拌洗脱，然后用聚乙二醇/ NaCl沉淀。结果，首先，发现浆果洗脱液中的酚含量与浆果样品中加标的大肠杆菌噬菌体MS2的检测之间存在弱关联。其次，更重要的是，观察到沉淀后形成的凝胶状沉淀可能捕获相当一部分病毒，无法进一步纯化和回收以进行检测，这表明来自浆果的病毒检测灵敏度低主要是由于浆果中果胶含量高且化学结构复杂。需要有针对性地解决这一问题的未来研究。