Coffee silverskin (CSS) is a coffee by-product obtained during the coffee roasting process and a potential source of proteins (14–19% protein content). In this study, conventional extraction (CE) and novel extraction methods were investigated for protein extraction from CSS. Optimum extraction pH and concentration for conventional acid and alkali extraction were studied. In addition, ultrasound-assisted extraction (UAE) and microwave-assisted extraction (MAE), which have been widely recognised as efficient and economic novel extraction techniques, were employed to enhance the protein extraction from CSS using alkaline and acid extraction. Among the extraction techniques studied, MAE was found to generate the highest yield of protein recovery, 43.53% of recovery with an average molecular weight of 6.64 kDa. Comparing with the conventional sequential extraction using NaOH followed by HCl at two different concentrations (0.2 M and 0.6 M), the recovery yields were significantly increased by 2.8-fold and 2.3-fold by applying UAE, and by 5.8-fold and 7-fold by applying MAE. The present work shows that UAE and MAE have potential to be rapid and effective tools for protein extraction from coffee silverskin. In this study, extracted proteins and raw material were characterised considering the molecular weight and the amino acid profile as well as its nutritional composition and its mineral contents.

咖啡银皮（CSS）是在咖啡烘焙过程中获得的咖啡副产品，并且是蛋白质的潜在来源（蛋白质含量为14-19％）。在这项研究中，常规提取（CE）和新颖的提取方法进行了研究从CSS提取蛋白质。研究了常规酸和碱提取的最佳提取pH和浓度。另外，被广泛认为是有效和经济的新颖提取技术的超声辅助提取（UAE）和微波辅助提取（MAE）被用于增强使用碱和酸提取的CSS蛋白质提取。在研究的提取技术中，发现MAE产生的蛋白质回收率最高，回收率为43.53％，平均分子量为6.64 kDa。与常规的依次使用NaOH依次萃取两种不同浓度（0.2 M和0.6 M）的HCl相比，使用UAE的回收率分别提高了2.8倍和2.3倍，并分别提高了5.8倍和7倍通过应用MAE。目前的工作表明，阿拉伯联合酋长国和MAE有潜力成为从咖啡银皮中提取蛋白质的快速而有效的工具。在这项研究中，提取的蛋白质和原料的特征在于分子量，氨基酸特征，营养成分和矿物质含量。目前的工作表明，阿拉伯联合酋长国和MAE有潜力成为从咖啡银皮中提取蛋白质的快速而有效的工具。在这项研究中，提取的蛋白质和原料的特征在于分子量，氨基酸特征，营养成分和矿物质含量。目前的工作表明，阿拉伯联合酋长国和MAE有潜力成为从咖啡银皮中提取蛋白质的快速有效的工具。在这项研究中，提取的蛋白质和原料的特征在于分子量，氨基酸特征，营养成分和矿物质含量。