Electric field stimulation has long been investigated with results supporting its therapeutic potential; however, its effects on insulin secreting cells has yet to be fully elucidated. Herein we explored the effects of physiological direct current (DC) electric field stimulation on the intracellular calcium dynamics of mouse derived βTC-6 insulinoma cells. This electrical stimulation resulted in an elevation in intracellular calcium along with a rise in calcium spiking activity. Further investigation indicated that the rise in intracellular calcium was mediated by an influx of calcium via L-type voltage gated calcium channels. Additionally, the effects of the electric field stimulation were able to induce insulin secretion in the absence of glucose stimulation. Given these results, DC electric field stimulation could be used as a non-invasive tool to modulate intracellular calcium dynamics and insulin secretion of β-cells for therapeutic application.

中文翻译：

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通过电场暴露改变 β 细胞钙动力学。

长期以来，人们一直在研究电场刺激，其结果支持其治疗潜力。然而，其对胰岛素分泌细胞的影响尚未完全阐明。在此，我们探讨了生理直流 (DC) 电场刺激对小鼠来源的 βTC-6 胰岛素瘤细胞的细胞内钙动力学的影响。这种电刺激导致细胞内钙的升高以及钙尖峰活动的增加。进一步的研究表明，细胞内钙的升高是由钙通过 L 型电压门控钙通道流入介导的。此外，电场刺激的效果能够在没有葡萄糖刺激的情况下诱导胰岛素分泌。鉴于这些结果，