Failure of intraoperative detection, early minimal lesion and microscopic residual tumor margins elimination causes metastatic diffusion and lethal recurrence. However, during surgical process, surgeons can only rely largely on palpation and visual examination. Intraoperative bioimaging with the aid of the second near-infrared fluorescent (NIR II FL) light has entered the surgical excision area to bridge the gap of preoperative bioimaging and intraoperative resection. Here, we demonstrate that the follicle-stimulating hormone peptide (FSHP) engineered NIR II downshifting nanoparticles (DSNPs@FSHP) selectively undergo efficient ovarian tumor targeting property. Owing to the special biocompatibility of nanoprobes, this strategy provided rapid body clearance and efficient tumor targeting with significantly tumor to background (T/B) ratio enhanced for surgical excision. Based on these, this strategy can successfully empower the detection and surgical removal for both ovarian tumor lesions and ovarian tumor margins by NIR II FL bioimaging.

术中检测失败、早期最小病变和显微镜下残留肿瘤边缘消除会导致转移扩散和致死性复发。然而，在手术过程中，外科医生在很大程度上只能依靠触诊和视觉检查。借助第二次近红外荧光（NIR II FL）光的术中生物成像已进入手术切除区域，以弥合术前生物成像和术中切除的差距。在这里，我们证明了卵泡刺激素肽 (FSHP) 工程 NIR II 降档纳米粒子 (DSNPs@FSHP) 选择性地具有有效的卵巢肿瘤靶向特性。由于纳米探针特殊的生物相容性，该策略提供了快速的身体清除和有效的肿瘤靶向，显着提高了手术切除的肿瘤与背景 (T/B) 比率。基于这些，该策略可以成功地通过 NIR II FL 生物成像检测和手术切除卵巢肿瘤病变和卵巢肿瘤边缘。