Livers from dab (Limanda limanda), plaice (Pleuronectes platessa) and flounder (Platichthys flesus) sampled from the Baltic Sea were used to determine the interaction of flatfish CYP1A enzymes with 2,4,6-trinitrotoluene (TNT) in vitro. Competitive inhibition of 7-ethoxyresorufin-O-deethylase (EROD) and 7-methoxyresorufin-O-deethylase (MROD) could be demonstrated for all three flatfish species. The highest inhibition of CYP1A activities was measured in liver samples of flounder resulting in a half maximal inhibitory concentration (IC₅₀) of 28.1 μM TNT. Due to their lower inhibition (EROD IC₅₀ 65.2 μM TNT, MROD IC₅₀ 40.3 μM TNT), dab liver samples were used to conduct in vitro metabolization experiments with TNT. The metabolization of TNT in fish was investigated with post-mitochondrial fractions (PMF) of dab liver as a model system after adding different cofactors. Rapid and time-dependent enzymatic degradation of TNT was observed. The concentrations of 4-amino-2,6-dinitrotoluene and 2-amino-4,6-dinitrotoluene increased in the samples over time. Additionally, 2,2,6,6-tetranitro-4,4-azoxytoluene was detected in one sample. The results of this study indicate that in vitro experiments are useful to investigate the xenobiotic metabolism of fish under controlled conditions prior to field studies. The metabolites found can serve as target compounds for marine monitoring of TNT contamination in munition dumpsites.

从DAB（肝脏黄盖鲽属黄盖鲽属），鲽鱼（鲽platessa）和比目鱼（Platichthys flesus从波罗的海采样的）被用来确定与2,4,6-三硝基甲苯（TNT）比目鱼CYP1A酶的相互作用在体外。可以证明所有三种比目鱼都具有竞争性抑制7-乙氧基间苯二酚-O-脱乙基酶（EROD）和7-甲氧基间苯二酚-O-脱乙基酶（MROD）的能力。在比目鱼的肝样品中测量到最高的CYP1A活性抑制作用，导致最大抑制浓度（IC ₅₀）为28.1μMTNT的一半。由于其较低的抑制作用（EROD IC ₅₀ 65.2μMTNT，MROD IC ₅₀使用40.3μMTNT），轻度肝样品进行TNT的体外代谢实验。在添加不同的辅因子后，以DAB肝的线粒体后级分（PMF）作为模型系统研究了鱼类中TNT的代谢。观察到TNT的快速且时间依赖性的酶促降解。样品中4-氨基-2,6-二硝基甲苯和2-氨基-4,6-二硝基甲苯的浓度随时间增加。另外，在一个样品中检测到2,2,6,6-四硝基-4,​​4-氮氧基甲苯。这项研究的结果表明，体外在田间研究之前，实验可用于研究鱼类在受控条件下的异生物代谢。发现的代谢物可作为目标化合物，用于海洋监测弹药堆场中的TNT污染。