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Efficient, fast and inexpensive bioassay to monitor benthic microalgae toxicity: Application to Ostreopsis species.
Aquatic Toxicology ( IF 4.1 ) Pub Date : 2020-04-22 , DOI: 10.1016/j.aquatox.2020.105485
Anne-Sophie Pavaux 1 , Eva Ternon 2 , Louison Dufour 1 , Sophie Marro 1 , Marin-Pierre Gémin 3 , Olivier P Thomas 4 , Rodolphe Lemée 1
Affiliation  

Even though HPLC-MS is commonly used to quantify the toxin content of Ostreopsis spp. cells, there is a need to develop easy-to-use toxicological tests to set thresholds during Ostreopsis spp. blooms. The crustacean Artemia has been widely used to evaluate the presence and toxicity of chemicals and biological contaminants and we anticipated that it could also be useful to test Ostreopsis spp. toxicity. Its relevance was first assessed by investigating the variability of the toxic effects among Ostreopsis spp. strains and throughout the dinoflagellate life cycle in combination with chemical analyses of the toxinic content by UHPLC-HRMS. After testing the toxicity of fractions prepared from Ostreopsis spp. cells, the known ova- and paly-toxins were not the only toxic metabolites to Artemia franciscana, indicating that other toxic compounds synthesized by Ostreopsis spp. still remain to be identified. To extend the bioassay to in situ monitoring, the toxicity of the benthic microalgal consortium was tested during a natural bloom of Ostreopsis cf. ovata in the NW Mediterranean Sea. The results highlight the accuracy and sensitivity of the ecotoxicological assay with Artemia franciscana to assess the toxicity of Ostreopsis spp. blooms.

中文翻译:

监测底栖微藻毒性的高效,快速和廉价的生物测定:在骨质疏松症物种中的应用。

即使HPLC-MS通常用于定量Ostreopsis spp的毒素含量。因此,有必要开发易于使用的毒理学测试以设定Ostreopsis spp期间的阈值。绽放。甲壳类Artemia已被广泛用于评估化学物质和生物污染物的存在和毒性,我们预计它也可用于测试Ostreopsis spp。毒性。它的相关性首先是通过研究Ostreopsis spp中毒作用的变化来评估的。菌株和整个鞭毛藻的生命周期,并结合通过UHPLC-HRMS对毒素含量进行化学分析。在测试由Ostreopsis spp制备的级分的毒性后。细胞中,已知的卵毒素和卵毒素并不是唯一的有毒的代谢产物,表明由Ostreopsis spp合成的其他有毒化合物。仍有待确定。为了将生物测定法扩展到原位监测,在Ostreopsis的自然开花过程中测试了底栖微藻财团的毒性。卵在西北地中海。结果强调了用法国大花蒿进行生态毒理学测定以评估骨质疏松症的毒性的准确性和敏感性。绽放。
更新日期:2020-04-23
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