Changes in serum inflammatory factor interleukin-6 levels and pathology of carotid vessel walls of rats with chronic periodontitis and diabetes mellitus after the periodontal intervention.,Saudi Journal of Biological Sciences

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Changes in serum inflammatory factor interleukin-6 levels and pathology of carotid vessel walls of rats with chronic periodontitis and diabetes mellitus after the periodontal intervention.
Saudi Journal of Biological Sciences Pub Date : 2020-04-23 , DOI: 10.1016/j.sjbs.2020.04.029
Danjun Chen ₁ , Liangjun Zhong ₁ , Yuan Li ₂ , Rui He ₁ , Cheng Ding ₁ , Xing Chen ₁ , Wenhai Lian ₁

Affiliation

Objective

The effects of the periodontal intervention on rats with type-II diabetes mellitus (T2DM) and chronic periodontitis (CP) were explored through observing the changes in carotid artery pathology and interleukin-6 (IL-6) levels.

Methods

The rats were randomly divided into 5 groups, i.e. group A (the normal control group), group B (the T2DM control group), group C (the CP control group), group D (the T2DM + CP group), and group E (the periodontal intervention T2DM + CP group). Blood samples of rats were collected from angular veins respectively at the following 5 time nodes: 1 week before the intervention (T1), 1 week after the intervention (T2), 3 weeks after the intervention (T3), 5 weeks after the intervention (T4), and 7 weeks after the intervention (T5); IL-6 concentrations before and after the intervention were determined by the enzyme-linked immunosorbent assay (ELISA), and the pathology of carotid arteries were observed by the Hematoxylin-Eosin (HE) stain.

Results

The pathological results of carotid arteries showed that the blood vessels of rats in group A were normal in morphology; most of the carotid artery vessel walls of rats in groups B, C, and D were significantly thickened and the fibers were disorderly arranged; the increased thickness of vessel walls of rats in group E was reduced, a small number of foam cells and inflammatory cells were observed, and the irregular arrangement of fibers was improved. In terms of the IL-6 concentrations, during the period of T1-T5, in groups B, C, and D, the IL-6 concentrations in rats were increased (P < 0.05); after the periodontal intervention, in group E, the IL-6 concentrations in rats were first increased then decreased (P < 0.05).

Conclusion

In terms of the long-term effects, periodontal intervention may reduce the inflammations of patients with diabetes mellitus and periodontitis and improve the lesions of carotid arteries.

中文翻译：

慢性牙周炎合并糖尿病大鼠牙周介入治疗后血清炎性因子IL-6水平及颈动脉血管壁病理变化[J].

客观的

通过观察颈动脉病理学和白细胞介素6（IL-6）水平的变化，探讨牙周干预对II型糖尿病（T2DM）和慢性牙周炎（CP）大鼠的影响。

方法

将大鼠随机分为5组，即A组（正常对照组）、B组（T2DM对照组）、C组（CP对照组）、D组（T2DM+CP组）、E组（牙周干预T2DM+CP组）。分别在以下5个时间节点采集大鼠角静脉血样：干预前1周（T1）、干预后1周（T2）、干预后3周（T3）、干预后5周（ T4) 和干预后 7 周 (T5)；采用酶联免疫吸附试验(ELISA)测定干预前后IL-6浓度，采用苏木精-伊红(HE)染色观察颈动脉病理。

结果

颈动脉病理结果显示，A组大鼠血管形态正常；B、C、D组大鼠颈动脉血管壁大部分明显增厚，纤维排列紊乱；E组大鼠血管壁增厚减少，可见少量泡沫细胞和炎性细胞，纤维排列不规则情况得到改善。在IL-6浓度方面，T1～T5期间，B、C、D组大鼠IL-6浓度升高（P < 0.05）；E组牙周干预后大鼠IL-6浓度先升高后降低（P < 0.05）。