Effects of sublethal phoxim exposure and lower food intake on nutrient metabolism in the midguts of Bombyx mori,Pesticide Biochemistry and Physiology

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Effects of sublethal phoxim exposure and lower food intake on nutrient metabolism in the midguts of Bombyx mori
Pesticide Biochemistry and Physiology ( IF 4.2 ) Pub Date : 2020-04-01 , DOI: 10.1016/j.pestbp.2020.104593
Zhiya Gu ₁ , Mengxue Li ₁ , Shuixiu Xia ₁ , Tingting Mao ₁ , Zhengting Lu ₁ , Jian Chen ₁ , Hui Wang ₁ , Jianwei Qu ₁ , Yilong Fang ₁ , Fanchi Li ₁ , Bing Li ₂

Affiliation

Silkworm (Bombyx mori) is an economically important insect. However, the survival of silkworms has been significantly affected by the assault of chemical pesticides on mulberry trees through aerial application and water currents. Phoxim is a broad-spectrum organophosphorus insecticide widely used in China. Currently, very little is known about the non-neuronal effects of sublethal exposure to phoxim. The purpose of this study was to investigate the non-neuronal effects of sublethal phoxim exposure in the silkworm midgut, with a focus on nutrient metabolism. After phoxim treatment, lipase activity in the silkworm was shown to be up-regulated at 24 h before a decreasing trend was seen. Meanwhile, α-amylase activity showed the opposite trend. The expression levels of mitochondrial respiratory chain-related genes were all up-regulated at 24 h before falling continuously. To ensure that the effects of phoxim on nutrient metabolism were not simply a consequence of a decrease in mulberry consumption, the silkworms were treated with a reduced-food diet before the digestive enzyme activities and the transcription levels of mitochondrial respiratory chain-related genes were analyzed. Our results showed that the patterns in the reduced-diet and phoxim-exposed silkworm were markedly different, suggesting the alterations in the phoxim-exposed silkworm cannot readily be explained by nutrient deprivation.

中文翻译：

亚致死辛硫磷暴露和低采食量对家蚕中肠营养代谢的影响

蚕（Bombyx mori）是一种重要的经济昆虫。然而，化学农药通过空中施药和水流对桑树的攻击，严重影响了蚕的生存。辛硫磷是一种广谱有机磷杀虫剂，在我国广泛使用。目前，对于亚致死性接触辛硫磷的非神经元影响知之甚少。本研究的目的是研究家蚕中肠亚致死辛硫磷暴露对非神经元的影响，重点是营养代谢。辛硫磷处理后，家蚕中的脂肪酶活性在 24 小时内上调，然后出现下降趋势。同时，α-淀粉酶活性表现出相反的趋势。线粒体呼吸链相关基因的表达水平均在24 h上调，然后持续下降。为了确保辛硫磷对营养代谢的影响不仅仅是桑树消耗量减少的结果，在消化酶活性和线粒体呼吸链相关基因的转录水平分析之前，对蚕进行了减食处理。 . 我们的研究结果表明，减少饮食的家蚕和接触辛硫磷的家蚕的模式明显不同，这表明接触辛硫磷的家蚕的变化不能轻易地用营养剥夺来解释。在分析消化酶活性和线粒体呼吸链相关基因的转录水平之前，对蚕进行了减食处理。我们的研究结果表明，减少饮食的家蚕和接触辛硫磷的家蚕的模式明显不同，这表明接触辛硫磷的家蚕的变化不能轻易地用营养剥夺来解释。在分析消化酶活性和线粒体呼吸链相关基因的转录水平之前，对蚕进行了减食处理。我们的研究结果表明，减少饮食的家蚕和接触辛硫磷的家蚕的模式明显不同，这表明接触辛硫磷的家蚕的变化不能轻易地用营养剥夺来解释。

更新日期：2020-04-01

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