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Orbencarb induces lethality and organ malformation in zebrafish embryos during development.
Comparative Biochemistry and Physiology C: Toxicology & Pharmacology ( IF 3.9 ) Pub Date : 2020-04-23 , DOI: 10.1016/j.cbpc.2020.108771 Jin-Young Lee 1 , Sunwoo Park 2 , Whasun Lim 3 , Gwonhwa Song 2
Comparative Biochemistry and Physiology C: Toxicology & Pharmacology ( IF 3.9 ) Pub Date : 2020-04-23 , DOI: 10.1016/j.cbpc.2020.108771 Jin-Young Lee 1 , Sunwoo Park 2 , Whasun Lim 3 , Gwonhwa Song 2
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Thiocarbamates are one of the components of pesticides that target weeds by inhibiting adenosine triphosphate (ATP) synthesis. Orbencarb, one of the isomeric thiocarbamates applied to wheat, maize, and soybean, has been found to have toxic effects on mammals and marine ecosystems. Although the toxicity ranges of orbencarb in different organisms are known, specific studies on the environmental contamination and harmful effects of orbencarb on non-target organisms are scarce. In this study, we observed that orbencarb induced embryotoxicity during zebrafish development as well as apoptosis and reactive oxygen species (ROS) production in the intestine. It was further observed that orbencarb decreased the viability of the embryos and simultaneously affected the heart rate and vessel formation. Orbencarb decreased the mRNA levels of ccnd1, ccne1, cdk2, and cdk6 and induced abnormal development of the eyes, brain, yolk sac, and spinal cord in zebrafish embryos. Orbencarb also hampered vasculogenesis in the zebrafish embryos by inhibiting the mRNA expression of flt1, flt4, kdr, and vegfc. Collectively, these results suggested that orbencarb is embryotoxic and disrupts the normal growth of zebrafish embryos by inducing the generation of ROS and hampering vasculogenesis.
中文翻译：
Orbencarb在发育过程中会引起斑马鱼胚胎的致死性和器官畸形。
硫代氨基甲酸酯是通过抑制三磷酸腺苷（ATP）合成来靶向杂草的农药成分之一。Orbencarb是应用于小麦，玉米和大豆的异构硫代氨基甲酸盐之一，现已发现对哺乳动物和海洋生态系统具有毒性作用。尽管奥本威在不同生物中的毒性范围是已知的，但关于奥本威对非目标生物的环境污染和有害影响的具体研究却很少。在这项研究中，我们观察到orbencarb诱导斑马鱼发育期间的胚胎毒性以及肠中的细胞凋亡和活性氧（ROS）产生。进一步观察到，奥本威降低了胚胎的活力，同时影响了心率和血管的形成。Orbencarb降低了ccnd1，ccne1，cdk2，和cdk6引起斑马鱼胚胎中的眼睛，大脑，卵黄囊和脊髓异常发育。Orbencarb还通过抑制flt1，flt4，kdr和vegfc的mRNA表达来抑制斑马鱼胚胎中的血管生成。总体而言，这些结果表明，本草威具有胚胎毒性，并通过诱导ROS的生成和阻碍血管生成而破坏了斑马鱼胚胎的正常生长。
更新日期：2020-04-23
中文翻译：
Orbencarb在发育过程中会引起斑马鱼胚胎的致死性和器官畸形。
硫代氨基甲酸酯是通过抑制三磷酸腺苷（ATP）合成来靶向杂草的农药成分之一。Orbencarb是应用于小麦，玉米和大豆的异构硫代氨基甲酸盐之一，现已发现对哺乳动物和海洋生态系统具有毒性作用。尽管奥本威在不同生物中的毒性范围是已知的，但关于奥本威对非目标生物的环境污染和有害影响的具体研究却很少。在这项研究中，我们观察到orbencarb诱导斑马鱼发育期间的胚胎毒性以及肠中的细胞凋亡和活性氧（ROS）产生。进一步观察到，奥本威降低了胚胎的活力，同时影响了心率和血管的形成。Orbencarb降低了ccnd1，ccne1，cdk2，和cdk6引起斑马鱼胚胎中的眼睛，大脑，卵黄囊和脊髓异常发育。Orbencarb还通过抑制flt1，flt4，kdr和vegfc的mRNA表达来抑制斑马鱼胚胎中的血管生成。总体而言，这些结果表明，本草威具有胚胎毒性，并通过诱导ROS的生成和阻碍血管生成而破坏了斑马鱼胚胎的正常生长。
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