Abstract Cryptolestes (Coleoptera: Laemophloeidae) are economically important pests, which damage stored products in granaries and mills. Because it is difficult to distinguish Cryptolestes species morphologically, we develop here a high sensitivity real-time PCR method for distinguishing five species of Cryptolestes (C. ferrugineus, C. pusillus, C. turcicus, C. pusilloides and C. capensis). The lower limit of DNA concentration required for species identification is 0.1 ng/μl for C. ferruginues and C. pusilloides, and 0.01 ng/μl for C. pusillus, C. turcicus, and C. capensis. This method successfully determined species assignments for previously unidentified Cryptolestes spp. Collected from the field and will facilitate Cryptolestes identification in the field of plant quarantine and stored product protection.

中文翻译：

---

基于 COI 条码区域的 5 种隐鱼的实时 PCR 鉴定

摘要 隐翅目（Coleoptera: Laemophloeidae）是一种重要的经济害虫，对粮仓和磨坊的储藏产品造成危害。由于在形态学上难以区分 Cryptolestes 物种，我们在这里开发了一种高灵敏度实时 PCR 方法来区分五种 Cryptolestes（C. ferrugineus、C. pusillus、C. turcicus、C. pusilloides 和 C. capensis）。物种鉴定所需的 DNA 浓度下限对于 C. ferruginues 和 C. pusilloides 为 0.1 ng/μl，对于 C. pusillus、C. turcicus 和 C. capensis 为 0.01 ng/μl。这种方法成功地确定了以前未识别的 Cryptolestes spp 的物种分配。从现场采集，将有助于植物检疫和贮藏产品保护领域的隐孢子虫鉴定。