Advancements in DNA nanotechnology and nanobioengineering allowed the use of DNA as a generic material, as well as a genetic material. Among various DNA-based materials, DNA hydrogels achieve a DNA-based network at the bulk scale. Notably, a recently developed enzyme-based fabrication method established a route to synthesize physically crosslinked networks from DNA. This new class of DNA networks paved the way for creating different forms of DNA materials by utilizing the characteristics of a biofunctional polymer. This Focus Review provides a brief overview of the enzyme-based fabrication method of physical DNA hydrogels and discusses the latest developments in technology and their applications. Recent advancements in DNA nanotechnology and nanobioengineering allowed the use of DNA as a generic material. Notably, an enzyme-based fabrication method of physical DNA hydrogels opened up a route to realize a novel class of DNA-based materials. Different forms of DNA materials with new applications were created by utilizing its unique characteristics. This Focus Review provides a brief overview of the enzyme-based fabrication method of physical DNA hydrogels and discusses the latest developments in the field.

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基于酶的物理 DNA 水凝胶制造：新材料和应用

DNA 纳米技术和纳米生物工程的进步允许使用 DNA 作为通用材料和遗传材料。在各种基于 DNA 的材料中，DNA 水凝胶在批量规模上实现了基于 DNA 的网络。值得注意的是，最近开发的一种基于酶的制造方法建立了一条从 DNA 合成物理交联网络的途径。这种新型 DNA 网络为利用生物功能聚合物的特性创造不同形式的 DNA 材料铺平了道路。本焦点综述简要概述了基于酶的物理 DNA 水凝胶制造方法，并讨论了技术及其应用的最新发展。DNA 纳米技术和纳米生物工程的最新进展允许使用 DNA 作为通用材料。尤其，一种基于酶的物理 DNA 水凝胶制造方法开辟了一条实现一类新型 DNA 材料的途径。利用其独特的特性，创造了具有新应用的不同形式的DNA材料。本焦点综述简要概述了基于酶的物理 DNA 水凝胶制造方法，并讨论了该领域的最新发展。