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Orange peel volatile oil: A green solvent for propolis extraction, enhanced α‐amylase inhibition activity
Flavour and Fragrance Journal ( IF 2.1 ) Pub Date : 2020-04-20 , DOI: 10.1002/ffj.3576 Şaban Keskin 1
Flavour and Fragrance Journal ( IF 2.1 ) Pub Date : 2020-04-20 , DOI: 10.1002/ffj.3576 Şaban Keskin 1
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Orange peel oil contains high amount of d‐limonene which is reported to have antidiabetic activity. Propolis contains many biologically active compounds like phenolic acids, flavonoids and terpenes. Because of its rich phenolic composition, antidiabetic activity of propolis has also been shown in literature. Consumption of raw propolis is limited because of its resinous nature. It should be extracted to convert it into consumable form. Ethanol is the most used solvent for this purpose. Ethanol is the limiting factor for propolis consumption either for religious concerns or being harmful for human health. There is an increasing need for new, compatible and healthier solvents for propolis extraction. This is the first paper describing the extraction of propolis with orange peel oil. 1:10 (g/v) ratio was used for extraction. Orange peel oil (OPO), orange peel oil propolis extract (OPOPE) and ethanol propolis extract (EPE) were separately analysed by using GC‐MS technique. Total phenolic content of oil and propolis extract was also carried out. Inhibition of α‐amylase from barley malt with new extract was also reported. It was found that OPOPE was rich in volatile compounds of propolis, phenolic acid derivatives and free fatty acids. It was observed that OPOPE showed higher inhibition on α‐amylase enzyme (IC50 0.196 mg/mL). It can be concluded that orange peel oil could be used as solvent for preparing propolis extract. Propolis extract obtained with orange peel oil could also be used as complementary medicine for the treatment of type 2 diabetes.
中文翻译：
橙皮挥发油：一种用于蜂胶提取的绿色溶剂，增强了α-淀粉酶抑制活性
桔皮油中含有大量的d-柠檬烯，据报道具有抗糖尿病活性。蜂胶含有许多生物活性化合物，例如酚酸，类黄酮和萜烯。由于其丰富的酚类成分，蜂胶的抗糖尿病活性也已在文献中显示。由于蜂胶的树脂性质，其消费量受到限制。应该将其提取以将其转换为消耗品形式。乙醇是为此目的最常用的溶剂。出于宗教考虑或对人体健康有害，乙醇是蜂胶消费的限制因素。蜂胶提取对新型，相容和健康的溶剂的需求不断增长。这是第一篇描述橙皮油提取蜂胶的论文。使用1:10（g / v）的比例进行提取。桔皮油（OPO），使用GC-MS技术分别分析了橘皮油蜂胶提取物（OPOPE）和乙醇蜂胶提取物（EPE）。还进行了油和蜂胶提取物中总酚含量的测定。还报道了用新提取物抑制大麦芽中的α-淀粉酶。发现OPOPE富含蜂胶，酚酸衍生物和游离脂肪酸的挥发性化合物。观察到OPOPE对α-淀粉酶（IC50 0.196 mg / mL）。可以得出结论，橙皮油可以用作制备蜂胶提取物的溶剂。用橙皮油获得的蜂胶提取物也可以用作治疗2型糖尿病的辅助药物。
更新日期：2020-04-20
中文翻译：
橙皮挥发油：一种用于蜂胶提取的绿色溶剂，增强了α-淀粉酶抑制活性
桔皮油中含有大量的d-柠檬烯，据报道具有抗糖尿病活性。蜂胶含有许多生物活性化合物，例如酚酸，类黄酮和萜烯。由于其丰富的酚类成分，蜂胶的抗糖尿病活性也已在文献中显示。由于蜂胶的树脂性质，其消费量受到限制。应该将其提取以将其转换为消耗品形式。乙醇是为此目的最常用的溶剂。出于宗教考虑或对人体健康有害，乙醇是蜂胶消费的限制因素。蜂胶提取对新型，相容和健康的溶剂的需求不断增长。这是第一篇描述橙皮油提取蜂胶的论文。使用1:10（g / v）的比例进行提取。桔皮油（OPO），使用GC-MS技术分别分析了橘皮油蜂胶提取物（OPOPE）和乙醇蜂胶提取物（EPE）。还进行了油和蜂胶提取物中总酚含量的测定。还报道了用新提取物抑制大麦芽中的α-淀粉酶。发现OPOPE富含蜂胶，酚酸衍生物和游离脂肪酸的挥发性化合物。观察到OPOPE对α-淀粉酶（IC50 0.196 mg / mL）。可以得出结论，橙皮油可以用作制备蜂胶提取物的溶剂。用橙皮油获得的蜂胶提取物也可以用作治疗2型糖尿病的辅助药物。
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