当前位置：
X-MOL 学术
›
Mol. Plant Pathol.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
The lre-miR159a-LrGAMYB pathway mediates resistance to grey mould infection in Lilium regale.
Molecular Plant Pathology ( IF 4.8 ) Pub Date : 2020-04-21 , DOI: 10.1111/mpp.12923 Xue Gao 1, 2 , Qian Zhang 1, 2 , Yu-Qian Zhao 1, 2 , Jie Yang 1, 2 , Heng-Bin He 1, 2 , Gui-Xia Jia 1, 2
Molecular Plant Pathology ( IF 4.8 ) Pub Date : 2020-04-21 , DOI: 10.1111/mpp.12923 Xue Gao 1, 2 , Qian Zhang 1, 2 , Yu-Qian Zhao 1, 2 , Jie Yang 1, 2 , Heng-Bin He 1, 2 , Gui-Xia Jia 1, 2
Affiliation
|
Grey mould is one of the most determinative factors of lily growth and plays a major role in limiting lily productivity. MicroRNA159 (miR159) is a highly conserved microRNA in plants, and participates in the regulation of plant development and stress responses. Our previous studies revealed that lre‐miR159a participates in the response of Lilium regale to Botrytis elliptica according to deep sequencing analyses; however, the response mechanism remains unknown. Here, lre‐miR159a and its target LrGAMYB gene were isolated from L. regale. Transgenic Arabidopsis overexpressing lre‐MIR159a exhibited larger leaves and smaller necrotic spots on inoculation with Botrytis than those of wild‐type and overexpressing LrGAMYB plants. The lre‐MIR159a overexpression also led to repressed expression of two targets of miR159, AtMYB33 and AtMYB65, and enhanced accumulation of hormone‐related genes, including AtPR1, AtPR2, AtNPR1, AtPDF1.2, and AtLOX for both the jasmonic acid and salicylic acid pathways. Moreover, lower levels of H2O2 and 
were observed in lre‐MIR159a transgenic Arabidopsis, which reduced the damage from reactive oxygen species accumulation. Taken together, these results indicate that lre‐miR159a positively regulates resistance to grey mould by repressing the expression of its target LrGAMYB gene and activating a defence response.
中文翻译：
lre-miR159a-LrGAMYB途径介导了对普通百合的灰霉病感染的抵抗力。
灰霉病是百合生长的最决定性因素之一，在限制百合生产力方面起着重要作用。MicroRNA159（miR159）是植物中高度保守的microRNA，并参与植物发育和胁迫响应的调控。我们以前的研究表明，根据深度测序分析，lre-miR159a参与了普通百合对椭圆形葡萄孢的反应。但是，响应机制仍然未知。在这里，lre-miR159a及其靶标LrGAMYB基因是从普通乳中分离得到的。过表达lre-MIR159a的转基因拟南芥在接种葡萄孢菌后表现出较大的叶片和较小的坏死斑比野生型和过表达的LrGAMYB植物要高。的LRE-MIR159a过表达还导致了miR159，两个目标压抑表达AtMYB33以及AtMYB65，以及增强的激素相关的基因，包括累积AtPR1，AtPR2，AtNPR1，AtPDF1.2和ATLOX用于茉莉酸和水杨酸既途径。此外，降低的高电平的情况下2 ö 2和中观察到LRE-MIR159a转基因拟南芥，减少了活性氧物种积累的危害。综上所述，这些结果表明，lre-miR159a通过抑制其靶标LrGAMYB基因的表达并激活防御反应而积极调节对灰霉病的抵抗力。
更新日期：2020-04-21
were observed in lre‐MIR159a transgenic Arabidopsis, which reduced the damage from reactive oxygen species accumulation. Taken together, these results indicate that lre‐miR159a positively regulates resistance to grey mould by repressing the expression of its target LrGAMYB gene and activating a defence response.
中文翻译：
lre-miR159a-LrGAMYB途径介导了对普通百合的灰霉病感染的抵抗力。
灰霉病是百合生长的最决定性因素之一，在限制百合生产力方面起着重要作用。MicroRNA159（miR159）是植物中高度保守的microRNA，并参与植物发育和胁迫响应的调控。我们以前的研究表明，根据深度测序分析，lre-miR159a参与了普通百合对椭圆形葡萄孢的反应。但是，响应机制仍然未知。在这里，lre-miR159a及其靶标LrGAMYB基因是从普通乳中分离得到的。过表达lre-MIR159a的转基因拟南芥在接种葡萄孢菌后表现出较大的叶片和较小的坏死斑比野生型和过表达的LrGAMYB植物要高。的LRE-MIR159a过表达还导致了miR159，两个目标压抑表达AtMYB33以及AtMYB65，以及增强的激素相关的基因，包括累积AtPR1，AtPR2，AtNPR1，AtPDF1.2和ATLOX用于茉莉酸和水杨酸既途径。此外，降低的高电平的情况下2 ö 2和
中观察到LRE-MIR159a转基因拟南芥，减少了活性氧物种积累的危害。综上所述，这些结果表明，lre-miR159a通过抑制其靶标LrGAMYB基因的表达并激活防御反应而积极调节对灰霉病的抵抗力。
京公网安备 11010802027423号