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Interaction of two strongly divergent archaellins stabilizes the structure of the Halorubrum archaellum.
MicrobiologyOpen ( IF 3.9 ) Pub Date : 2020-04-21 , DOI: 10.1002/mbo3.1047 Mikhail G Pyatibratov 1 , Alexey S Syutkin 1 , Tessa E F Quax 2 , Tatjana N Melnik 1 , R Thane Papke 3 , Johann Peter Gogarten 3 , Igor I Kireev 4 , Alexey K Surin 1, 5, 6 , Sergei N Beznosov 1 , Anna V Galeva 1 , Oleg V Fedorov 1
MicrobiologyOpen ( IF 3.9 ) Pub Date : 2020-04-21 , DOI: 10.1002/mbo3.1047 Mikhail G Pyatibratov 1 , Alexey S Syutkin 1 , Tessa E F Quax 2 , Tatjana N Melnik 1 , R Thane Papke 3 , Johann Peter Gogarten 3 , Igor I Kireev 4 , Alexey K Surin 1, 5, 6 , Sergei N Beznosov 1 , Anna V Galeva 1 , Oleg V Fedorov 1
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Halophilic archaea from the genus Halorubrum possess two extraordinarily diverged archaellin genes, flaB1 and flaB2. To clarify roles for each archaellin, we compared two natural Halorubrum lacusprofundi strains: One of them contains both archaellin genes, and the other has the flaB2 gene only. Both strains synthesize functional archaella; however, the strain, where both archaellins are present, is more motile. In addition, we expressed these archaellins in a Haloferax volcanii strain from which the endogenous archaellin genes were deleted. Three Hfx. volcanii strains expressing Hrr. lacusprofundi archaellins produced functional filaments consisting of only one (FlaB1 or FlaB2) or both (FlaB1/FlaB2) archaellins. All three strains were motile, although there were profound differences in the efficiency of motility. Both native and recombinant FlaB1/FlaB2 filaments have greater thermal stability and resistance to low salinity stress than single‐component filaments. Functional supercoiled Hrr. lacusprofundi archaella can be composed of either single archaellin: FlaB2 or FlaB1; however, the two divergent archaellin subunits provide additional stabilization to the archaellum structure and thus adaptation to a wider range of external conditions. Comparative genomic analysis suggests that the described combination of divergent archaellins is not restricted to Hrr. lacusprofundi, but is occurring also in organisms from other haloarchaeal genera.
中文翻译：
两种高度发散的古细菌的相互作用稳定了哈洛布鲁姆古细菌的结构。
从该属嗜盐古Halorubrum具有两个非常分歧archaellin基因，flaB1和flaB2。为了弄清每种古菌素的作用，我们比较了两种天然Halorubrum lacusprofundi菌株：其中一种含有古菌素基因，另一种仅含有flaB2基因。两种菌株都合成功能古细菌。但是，这两种古细菌都存在的菌株更能运动。另外，我们在Haloferax volcanii菌株中表达了这些古菌素，从中删除了内生古菌素基因。三个Hfx。表达Hrr的volcanii菌株。乳杆菌古细菌素产生仅由一个古细菌素（FlaB1或FlaB2）或两者（FlaB1 / FlaB2）组成的功能性细丝。尽管运动效率有显着差异，但这三种菌株均能运动。天然和重组FlaB1 / FlaB2细丝均比单组分细丝具有更高的热稳定性和对低盐度的抵抗力。功能超螺旋HRR。procusi lacusprofundi archaella可以由单个archaellin组成：FlaB2或FlaB1；然而，两个不同的古细菌亚单位为古细菌的结构提供了额外的稳定性，从而适应了更大范围的外部条件。比较基因组分析表明，所描述的不同古细菌的组合不限于HRr。lacusprofundi， 但也存在于其他卤古细菌属的生物中。
更新日期：2020-04-21
中文翻译：
两种高度发散的古细菌的相互作用稳定了哈洛布鲁姆古细菌的结构。
从该属嗜盐古Halorubrum具有两个非常分歧archaellin基因，flaB1和flaB2。为了弄清每种古菌素的作用，我们比较了两种天然Halorubrum lacusprofundi菌株：其中一种含有古菌素基因，另一种仅含有flaB2基因。两种菌株都合成功能古细菌。但是，这两种古细菌都存在的菌株更能运动。另外，我们在Haloferax volcanii菌株中表达了这些古菌素，从中删除了内生古菌素基因。三个Hfx。表达Hrr的volcanii菌株。乳杆菌古细菌素产生仅由一个古细菌素（FlaB1或FlaB2）或两者（FlaB1 / FlaB2）组成的功能性细丝。尽管运动效率有显着差异，但这三种菌株均能运动。天然和重组FlaB1 / FlaB2细丝均比单组分细丝具有更高的热稳定性和对低盐度的抵抗力。功能超螺旋HRR。procusi lacusprofundi archaella可以由单个archaellin组成：FlaB2或FlaB1；然而，两个不同的古细菌亚单位为古细菌的结构提供了额外的稳定性，从而适应了更大范围的外部条件。比较基因组分析表明，所描述的不同古细菌的组合不限于HRr。lacusprofundi， 但也存在于其他卤古细菌属的生物中。
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