The highly diverse chemical structures of lipids make their analysis directly from biological tissue sections extremely challenging. Here, we report the in situ mapping and identification of lipids in a freshwater crustacean Gammarus fossarum using matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) in combination with an additional separation dimension using ion mobility spectrometry (IMS). The high‐resolution trapped ion mobility spectrometry (TIMS) allowed efficient separation of isobaric/isomeric lipids showing distinct spatial distributions. The structures of the lipids were further characterized by MS/MS analysis. It is demonstrated that MALDI MSI with mobility separation is a powerful tool for distinguishing and localizing isobaric/isomeric lipids.

中文翻译：

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通过MALDI离子淌度分离质谱法原位等压脂质作图。

脂质的高度多样化的化学结构使得直接从生物组织切片进行分析非常具有挑战性。在这里，我们报告了使用基质辅助激光解吸/电离（MALDI）质谱成像（MSI）结合使用离子迁移谱（IMS）的附加分离尺寸对淡水甲壳类Gammarus fossarum中脂质的原位作图和鉴定。高分辨率捕获离子迁移谱法（TIMS）可以有效分离显示出独特空间分布的同量异位/异构脂质。通过MS / MS分析进一步表征脂质的结构。事实证明，具有迁移率分离的MALDI MSI是区分和定位同量异位/异构脂质的强大工具。