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Nanoemulsions with oleoresin of Copaifera reticulata (Leguminosae) improve anthelmintic efficacy in the control of monogenean parasites when compared to oleoresin without nanoformulation.
Journal of Fish Diseases ( IF 2.2 ) Pub Date : 2020-04-21 , DOI: 10.1111/jfd.13168
Dayna Filocreão Malheiros 1 , Icaro Rodrigues Sarquis 2 , Irlon Maciel Ferreira 2 , Patrick Delgado Mathews 3 , Omar Mertins 4 , Marcos Tavares-Dias 1, 5
Affiliation  

This study compared the in vitro anthelmintic activity of Copaifera reticulata oleoresin (200, 400, 600, 800 and 1,000 mg/L) and of nanoemulsions prepared with this oleoresin (50, 100, 150, 200 and 250 mg/L) against monogeneans on the gills of Colossoma macropomum. The major compounds present in the oleoresin of C. reticulata were γ-macrocarpene (14.2%), α-bergamotene (13.6%), β-selinene (13.4%) and β-caryophyllene (11.7%). All concentrations of the nanoemulsion and the oleoresin without nanoformulation showed anthelmintic efficacy against monogeneans, and higher concentrations led to more rapid parasite mortality. Structural damages to the tegument of the parasites exposed to C. reticulata oleoresin were observed with scanning electron microscopy. At two hours of exposure, fish showed 100% tolerance to all nanoemulsion concentrations used in the in vitro assays, whereas 100% mortality was shown in the fish exposed to the oleoresin without nanoformulation after one hour. The results of this study suggest that nanoemulsions with oleoresin of C. reticulata have advantages in the control and treatment of monogenean infections in C. macropomum when compared to the oleoresin without nanoformulation. In addition, since nanoemulsions with the C. reticulata oleoresin are safe to control monogeneans, the efficacy of these nanoformulations may be assayed in therapeutic baths to treat C. macropomum infected by monogeneans.

中文翻译:

与无纳米配方的油性树脂相比,含网状油菜(豆科)的油性树脂的纳米乳剂可提高驱虫效果,以控制单基因寄生虫。

这项研究比较了网纹油菜油树脂(200、400、600、800和1,000 mg / L)和用该油树脂制备的纳米乳剂(50、100、150、200和250 mg / L)对单基因菌的体外驱虫活性。巨大巨Co的g。网纹梭菌的油性树脂中存在的主要化合物为γ-大麦烯(14.2%),α-佛手柑烯(13.6%),β-selinene(13.4%)和β-石竹烯(11.7%)。所有浓度的纳米乳液和不含树脂的油性树脂均显示出抗单基因的驱虫药功效,浓度越高,寄生虫死亡率越高。用扫描电子显微镜观察到暴露于网状油松脂蛋白的寄生虫的皮甲的结构破坏。在暴露两个小时后 鱼类对体外测定中使用的所有纳米乳剂浓度表现出100%的耐受性,而一小时后暴露于油脂树脂而不进行纳米配方的鱼类显示出100%的死亡率。这项研究的结果表明,与无纳米配方的油性树脂相比,含网状油酸油性树脂的纳米乳剂在控制和治疗巨球藻单基因感染方面具有优势。另外,由于用网状油菜油质树脂的纳米乳剂可以安全地控制单基因菌,因此可以在治疗浴中测定这些纳米制剂的功效,以治疗被单基因菌感染的大型梭状芽胞杆菌。与没有纳米制剂的油性树脂相比,网纹菌在大果念珠菌单基因感染的控制和治疗方面具有优势。另外,由于用网状油菜油质树脂的纳米乳剂可以安全地控制单基因菌,因此可以在治疗浴中测定这些纳米制剂的功效,以治疗被单基因菌感染的大型梭状芽胞杆菌。与没有纳米制剂的油性树脂相比,网纹菌在控制和治疗大型梭状芽胞杆菌中具有优势。另外,由于用网状油菜油质树脂的纳米乳剂可以安全地控制单基因菌,因此可以在治疗浴中测定这些纳米制剂的功效,以治疗被单基因菌感染的大型梭状芽胞杆菌。
更新日期:2020-04-21
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