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Capacitance Polypyrrole‐based Impedimetric Immunosensor for Interleukin‐10 Cytokine Detection
Electroanalysis ( IF 2.7 ) Pub Date : 2020-04-09 , DOI: 10.1002/elan.201900633 Faiza Nessark 1, 2, 3 , Mohamed Eissa 1, 4 , Abdoullatif Baraket 1 , Nadia Zine 1 , Belkacem Nessark 2 , Ahmed Zouaoui 5 , Joan Bausells 6 , Abdelhamid Errachid 1
Electroanalysis ( IF 2.7 ) Pub Date : 2020-04-09 , DOI: 10.1002/elan.201900633 Faiza Nessark 1, 2, 3 , Mohamed Eissa 1, 4 , Abdoullatif Baraket 1 , Nadia Zine 1 , Belkacem Nessark 2 , Ahmed Zouaoui 5 , Joan Bausells 6 , Abdelhamid Errachid 1
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In the present study, we developed a novel label‐free capacitance impedimetric immunosensor based on the immobilization of the human monoclonal antibody anti‐interleukin‐10 (anti‐IL‐10 mAb) onto polypyrrole (PPy)‐modified silicon nitride (Si3N4) substrates. The immunosensor was used for the detection of the recombinant interleukin‐10 antigen (rh IL‐10) that may be secreted in patients at the early stage of inflammation. The immunosensor was created by chemical deposition of PPy conducting layer on pyrrole−silane (SPy)‐treated Si/SiO2/Si3N4 substrates (Si/SiO2/Si3N4−SPy), followed by anti‐IL‐10 mAb immobilization through carboxyl‐functionalized diazonium (CMA) protocol and carbodiimide chemistry. The surface characterization and the biofunctionalization steps were characterized by SEM, FTIR and cyclic voltammetry (CV) while the detection process was carried out by using electrochemical impedance spectroscopy (EIS) analyses. The created immunosensor showed two linear fittings (R2=0.999) for the detection of rh IL‐10 within the concentration range from 1–50 pg/mL. It exhibited high sensitivity (0.1128 (pg/mL)−1) with a very low limit of detection (LOD)=0.347 pg/mL, more particularly, at the low concentration range (1–10 pg/mL). Thus, this developed polypyrrole‐based immunosensor represents a promising strategy for creation of miniaturized label‐free, fast and highly sensitive biosensors for diagnosis of inflammation biomarkers at very low concentrations with reduced cost.
中文翻译：
用于白介素10细胞因子检测的基于电容聚吡咯的阻抗免疫传感器
在本研究中，我们基于将人类单克隆抗体抗白介素10（抗IL-10 mAb）固定在聚吡咯（PPy）改性的氮化硅（Si 3 N ）上开发了一种新型的无标记电容阻抗免疫传感器。4）基材。免疫传感器用于检测炎症早期患者中可能分泌的重组白介素-10抗原（rh IL-10）。免疫传感器是通过在吡咯硅烷（SPy）处理的Si / SiO 2 / Si 3 N 4衬底（Si / SiO 2 / Si 3 N 4-SPy），然后通过羧基官能化重氮（CMA）方案和碳二亚胺化学固定抗IL-10 mAb。通过SEM，FTIR和循环伏安法（CV）对表面表征和生物功能化步骤进行了表征，同时使用电化学阻抗谱（EIS）分析进行了检测过程。所创建的免疫传感器在1-50 pg / mL的浓度范围内显示了两个线性拟合（R 2 = 0.999）用于检测rh IL-10。它表现出高灵敏度（0.1128（pg / mL）-1）的检测限（LOD）仅为0.347 pg / mL，特别是在低浓度范围（1–10 pg / mL）时。因此，这种开发的基于聚吡咯的免疫传感器代表了一种有前途的策略，可用于创建无标记的，快速且高度灵敏的小型生物传感器，以非常低的浓度诊断炎症生物标志物，从而降低了成本。
更新日期：2020-04-09
中文翻译：
用于白介素10细胞因子检测的基于电容聚吡咯的阻抗免疫传感器
在本研究中，我们基于将人类单克隆抗体抗白介素10（抗IL-10 mAb）固定在聚吡咯（PPy）改性的氮化硅（Si 3 N ）上开发了一种新型的无标记电容阻抗免疫传感器。4）基材。免疫传感器用于检测炎症早期患者中可能分泌的重组白介素-10抗原（rh IL-10）。免疫传感器是通过在吡咯硅烷（SPy）处理的Si / SiO 2 / Si 3 N 4衬底（Si / SiO 2 / Si 3 N 4-SPy），然后通过羧基官能化重氮（CMA）方案和碳二亚胺化学固定抗IL-10 mAb。通过SEM，FTIR和循环伏安法（CV）对表面表征和生物功能化步骤进行了表征，同时使用电化学阻抗谱（EIS）分析进行了检测过程。所创建的免疫传感器在1-50 pg / mL的浓度范围内显示了两个线性拟合（R 2 = 0.999）用于检测rh IL-10。它表现出高灵敏度（0.1128（pg / mL）-1）的检测限（LOD）仅为0.347 pg / mL，特别是在低浓度范围（1–10 pg / mL）时。因此，这种开发的基于聚吡咯的免疫传感器代表了一种有前途的策略，可用于创建无标记的，快速且高度灵敏的小型生物传感器，以非常低的浓度诊断炎症生物标志物，从而降低了成本。
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