Alpha‐ and beta‐tubulin dimers polymerize into protofilaments that associate laterally to constitute a hollow tube, the microtubule. A dynamic network of interlinking filaments forms the microtubule cytoskeleton, which maintains the structure of cells and is key to various cellular processes including cell division, cell migration, and intracellular transport. Individual microtubules have an identity that depends on the differential integration of specific alpha‐ and beta‐tubulin isotypes and is further specified by a variety of posttranslational modifications (PTMs). It is barely understood to which extent neighboring microtubules differ in their tubulin composition or whether specific tubulin isotypes cluster along the polymer. Furthermore, our knowledge about the spatio‐temporal expression patterns of tubulin isotypes is limited, not at least due to the lack of antibodies or antibody cross‐reactivities. Here, we asked which alpha‐ and beta‐tubulin mRNAs and proteins are expressed in developing hippocampal neuron cultures and ex vivo brain tissue lysates. Using heterologous expression of GFP‐tubulin fusion proteins, we systematically tested antibody‐specificities against various tubulin isotypes. Our data provide quantitative information about tubulin expression levels in the mouse brain and classify tubulin isotypes during pre‐ and postnatal development.

中文翻译：

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α-和β-微管蛋白同种型在大脑发育过程中差异表达

α-和β-微管蛋白二聚体聚合成原丝，这些原丝横向结合，形成一个中空管，即微管。相互连接的细丝的动态网络形成微管细胞骨架，它维持细胞的结构并且是包括细胞分裂、细胞迁移和细胞内运输在内的各种细胞过程的关键。单个微管的特性取决于特定 α 和 β 微管蛋白同种型的差异整合，并通过各种翻译后修饰 (PTM) 进一步指定。几乎不了解相邻微管在其微管蛋白组成方面的差异程度，或者特定的微管蛋白同种型是否沿着聚合物聚集。此外，我们对微管蛋白同种型的时空表达模式的了解是有限的，至少不是因为缺乏抗体或抗体交叉反应。在这里，我们询问了在发育中的海马神经元培养物和离体脑组织裂解物中表达了哪些 α 和 β 微管蛋白 mRNA 和蛋白质。利用 GFP-微管蛋白融合蛋白的异源表达，我们系统地测试了针对各种微管蛋白同种型的抗体特异性。我们的数据提供了关于小鼠大脑中微管蛋白表达水平的定量信息，并在产前和产后发育过程中对微管蛋白同种型进行了分类。我们系统地测试了针对各种微管蛋白同种型的抗体特异性。我们的数据提供了关于小鼠大脑中微管蛋白表达水平的定量信息，并在产前和产后发育过程中对微管蛋白同种型进行了分类。我们系统地测试了针对各种微管蛋白同种型的抗体特异性。我们的数据提供了有关小鼠大脑中微管蛋白表达水平的定量信息，并在产前和产后发育过程中对微管蛋白同种型进行了分类。