A common theme in organogenesis is how the final structure of organs emerge from epithelial tube structures, with the formation of the neural tube being one of the best examples. Two types of cell movements co‐occur during neural tube closure involving the migration of cells toward the midline of the embryo (mediolateral intercalation or convergent extension) as well as the deep movement of cells from inside the embryo to the outside of the lateral side of the neural plate (radial intercalation). Failure of either type of cell movement will prevent neural tube closure, which can produce a range of neural tube defects (NTDs), a common congenital disease in humans. Numerous studies have identified signaling pathways that regulate mediolateral intercalation during neural tube closure. Less understood are the pathways that govern radial intercalation. Using the Xenopus laevis system, our group reported the identification of transient receptor potential (TRP) channels, TRPM6 and TRPM7, and the Mg²⁺ ion they conduct, as novel and key factors regulating both mediolateral and radial intercalation during neural tube closure. Here we broadly discuss tubulogenesis and cell intercalation from the perspective of neural tube closure and the respective roles of TRPM7 and TRPM6 in this critical embryonic process.

中文翻译：

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TRPM6 和 TRPM7：脊椎动物胚胎发育过程中细胞嵌入的新参与者。

器官发生的一个共同主题是器官的最终结构如何从上皮管​​结构中产生，神经管的形成就是最好的例子之一。神经管闭合过程中同时发生两种类型的细胞运动，包括细胞向胚胎中线迁移（中间外侧嵌入或会聚延伸）以及细胞从胚胎内部向胚胎外侧的深层运动。神经板（径向嵌入）。任何一种类型的细胞运动失败都会阻止神经管闭合，从而导致一系列神经管缺陷（NTD），这是人类常见的先天性疾病。许多研究已经确定了在神经管闭合过程中调节内侧嵌入的信号通路。不太了解的是控制径向插层的途径。在非洲爪蟾系统中，我们小组报道了瞬时受体电位（TRP）通道TRPM6和TRPM7以及它们传导的Mg ²⁺离子的鉴定，作为神经管闭合过程中调节内侧和径向嵌入的新颖且关键的因素。在这里，我们从神经管闭合的角度广泛讨论肾小管发生和细胞嵌入，以及 TRPM7 和 TRPM6 在这一关键胚胎过程中各自的作用。