The effect of IFN‐β 1a on expression of MDA5 and RIG‐1 in multiple sclerosis patients,Biotechnology and Applied Biochemistry

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The effect of IFN‐β 1a on expression of MDA5 and RIG‐1 in multiple sclerosis patients
Biotechnology and Applied Biochemistry ( IF 3.2 ) Pub Date : 2020-04-20 , DOI: 10.1002/bab.1920
Gholamreza Asadikaram _{1,

2,

3} , Hossein Ali Ebrahimi Meimand ₁ , Saam Noroozi _{2,

4} , Mojgan Sanjari ₅ , Nahid Zainodini ₆ , Mohammad Kazemi Arababadi _{6,

7}

Affiliation

The aims of this study were to compare mRNA levels of melanoma differentiation‐associated protein 5 (MDA5) and retinoic acid‐inducible gene 1 (RIG‐1) in multiple sclerosis (MS) patients in comparison to the healthy controls as well as investigating the effects of IFN‐β 1a on the expression of these molecules. In this study, mRNA levels of MDA5 and RIG‐1 in peripheral leukocytes of 30 new cases of MS patients and 35 healthy controls were evaluated using the real‐time‐PCR method. mRNA levels of MDA5 and RIG‐1 were determined in the MS patients 6 months after treatment with standard doses of IFN‐β 1a. mRNA levels of MDA5 and RIG‐1 were significantly decreased in the MS patients in comparison to the healthy controls. The analysis also revealed that IFN‐β 1a therapy leads to the upregulation of RIG‐1, but not MDA5, in the total MS patients and the female group. MS patients suffer from insufficient expression of MDA5 and RIG‐1, and IFN‐β 1a therapy results in the upregulation of RIG‐1 in the patients, especially in the female patients. Thus, it seems that IFN‐β 1a not only decreased pathogenic inflammatory responses but also modulated the expression of RIG‐1 to protect the patients from infectious diseases and upregulation of IFN‐I in a positive feedback.

中文翻译：

IFN-β1a对多发性硬化症患者MDA5和RIG-1表达的影响

本研究的目的是比较多发性硬化症 (MS) 患者与健康对照者中黑色素瘤分化相关蛋白 5 (MDA5) 和视黄酸诱导基因 1 (RIG-1) 的 mRNA 水平，并调查IFN-β 1a 对这些分子表达的影响。在本研究中，使用实时 PCR 方法评估了 30 名新发 MS 患者和 35 名健康对照者外周白细胞中 MDA5 和 RIG-1 的 mRNA 水平。在接受标准剂量的 IFN-β 1a 治疗 6 个月后，测定 MS 患者的 MDA5 和 RIG-1 mRNA 水平。与健康对照相比，MS 患者的 MDA5 和 RIG-1 mRNA 水平显着降低。分析还显示，在所有 MS 患者和女性组中，IFN-β 1a 治疗会导致 RIG-1 上调，但不会上调 MDA5。 MS患者MDA5和RIG-1表达不足，IFN-β1a治疗导致患者RIG-1上调，尤其是女性患者。因此，IFN-β 1a 似乎不仅可以减少致病性炎症反应，还可以调节 RIG-1 的表达以保护患者免受感染性疾病的侵害，并以正反馈的方式上调 IFN-I。

更新日期：2020-04-20

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