Natural products extracted from plants are an alternative method for controlling postharvest citrus blue mold, caused by Penicillium italicum (P. italicum). In this study, RNA sequence analysis was used to investigate the underlying anti-fungal mechanism of flavonoids from Sedum aizoon L. (FSAL) on P. italicum. Significant differences in 3592 genes were observed, including 2507 up-regulated and 1085 down-regulated genes between the FSAL-treated and the control groups. Furthermore, the GO and KEGG analysis results indicated that FSAL inhibited genes related to the integral components of membrane, oxidation-reduction process, mitochondrion, ribosome, and amino acid metabolism. In the presence of FSAL, the cellular constituents, including DNA and RNA were leaked from hyphae of P. italicum. Reactive oxygen species (ROS) production in P. italicum was also determined with a significant concentration-effect under the treatment of FSAL. Thus, we speculate that the inhibitory activity of FSAL on P. italicum is mainly achieved by damaging the structure of the cell membrane and cell wall, disrupting the process of mitochondrial respiratory metabolism, protein biosynthesis, and amino acid metabolism, leading to cell death. The present study provided a global perspective on the molecular mechanism of FSAL on P. italicum through transcriptome analysis, which may help develop a novel plant-derived anti-fungal agent for the blue mold of citrus.

中文翻译：

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青霉菌响应景天黄酮类化合物的转录组分析。

从植物中提取的天然产物是控制由意大利青霉菌 (P. italicum) 引起的收获后柑橘蓝霉菌的另一种方法。在这项研究中，RNA 序列分析用于研究来自 Sedum aizoon L. (FSAL) 的黄酮类化合物对 P. italicum 的潜在抗真菌机制。观察到 3592 个基因的显着差异，包括 FSAL 处理组和对照组之间的 2507 个上调基因和 1085 个下调基因。此外，GO和KEGG分析结果表明，FSAL抑制与膜、氧化还原过程、线粒体、核糖体和氨基酸代谢等组成部分相关的基因。在 FSAL 存在的情况下，细胞成分，包括 DNA 和 RNA 从 P. italicum 的菌丝中泄漏。P. 中活性氧 (ROS) 的产生。在 FSAL 处理下，italicum 也具有显着的浓度效应。因此，我们推测FSAL对P. italicum的抑制活性主要是通过破坏细胞膜和细胞壁的结构，破坏线粒体呼吸代谢、蛋白质生物合成和氨基酸代谢过程，导致细胞死亡来实现的。本研究通过转录组分析为 P. italicum 上 FSAL 的分子机制提供了一个全局视角，这可能有助于开发一种新型植物源性抗柑橘蓝霉病的抗真菌剂。扰乱线粒体呼吸代谢、蛋白质生物合成和氨基酸代谢过程，导致细胞死亡。本研究通过转录组分析为 P. italicum 上 FSAL 的分子机制提供了一个全局视角，这可能有助于开发一种新型植物源性抗柑橘蓝霉病的抗真菌剂。扰乱线粒体呼吸代谢、蛋白质生物合成和氨基酸代谢过程，导致细胞死亡。本研究通过转录组分析为 P. italicum 上 FSAL 的分子机制提供了一个全局视角，这可能有助于开发一种新型植物源性抗柑橘蓝霉病的抗真菌剂。