Background Pyridoxine (PDX; vitamin B 6 ), is an essential vitamin. PDX deficiency induces various symptoms, and when PDX is misused it acts as a neurotoxicant, inducing severe sensory neuropathy. Results To assess the possibility of creating a reversible sensory neuropathy model using dogs, 150 mg/kg of PDX was injected subcutaneously into dogs for 7 days and body weight measurements, postural reaction assessments, and electrophysiological recordings were obtained. In addition, the morphology of dorsal root ganglia (DRG) and changes in glial fibrillary acidic protein (GFAP) immunoreactive satellite glial cells and ionized calcium-binding adapter molecule 1 (Iba-1) immunoreactive microglia/macrophages were assessed at 1 day, 1 week, and 4 weeks after the last PDX treatment. During the administration period, body weight and proprioceptive losses occurred. One day after the last PDX treatment, electrophysiological recordings showed the absence of the H-reflex in the treated dogs. These phenomena persisted over the four post-treatment weeks, with the exception of body weight which recovered to the pre-treatment level. Staining (CV and HE) results revealed significant losses of large-sized neurons in the DRG at 1 day and 1 week after PDX treatment cessation, but the losses were recovered at 4 weeks post-treatment. The Iba-1 and GFAP immunohistochemistry results showed pronounced increases in reactive microglia/macrophage and satellite glial cell at 1 day and 1 week, respectively, after the last PDX treatment, and thereafter, immunoreactivity decreased with increasing time after PDX treatment. Conclusions The results suggest that PDX-induced neuropathy is reversible in dogs; thus, dogs can be considered a good experimental model for research on neuropathy.

中文翻译：

---

吡哆醇诱导犬背根神经节的神经病理学变化

背景 吡哆醇（PDX；维生素 B 6 ）是一种必需的维生素。PDX 缺乏会引起各种症状，当 PDX 被滥用时，它会作为一种神经毒剂，诱发严重的感觉神经病变。结果 为了评估使用狗创建可逆感觉神经病模型的可能性，将 150 mg/kg 的 PDX 皮下注射到狗体内 7 天，并获得体重测量、姿势反应评估和电生理记录。此外，在第 1 天、第 1 天评估了背根神经节 (DRG) 的形态以及胶质纤维酸性蛋白 (GFAP) 免疫反应性卫星神经胶质细胞和离子钙结合接头分子 1 (Iba-1) 免疫反应性小胶质细胞/巨噬细胞的变化。一周和最后一次 PDX 治疗后 4 周。在执政期间，发生体重和本体感觉损失。在最后一次 PDX 治疗后一天，电生理记录显示治疗犬没有 H 反射。除了体重恢复到治疗前的水平外，这些现象在治疗后的四个星期内持续存在。染色（CV 和 HE）结果显示，在 PDX 治疗停止后 1 天和 1 周，DRG 中大尺寸神经元的显着损失，但这些损失在治疗后 4 周恢复。Iba-1 和 GFAP 免疫组织化学结果显示，在最后一次 PDX 治疗后第 1 天和第 1 周，反应性小胶质细胞/巨噬细胞和卫星神经胶质细胞显着增加，此后，免疫反应性随着 PDX 治疗时间的增加而降低。结论 结果表明，PDX 诱导的神经病变在犬中是可逆的；因此，狗可以被认为是研究神经病的一个很好的实验模型。