Spinal cord injuries (SCI) normally disrupt the long axonal tracts of the spinal cord and cause permanent neurological deficits, for which there is currently a lack of effective therapeutic methods. Biomaterial-based regenerative medicine is a pivotal strategy to induce axonal regeneration through delivery of biophysical and/or biochemical regulatory cues by biomaterials. We previously fabricated a hierarchically aligned fibrin hydrogel (AFG) that could promote neurogenic differentiation of stem cells in vitro and has been successfully applied for peripheral nerve and spinal cord regeneration in rats. In this study, AFG was used to repair a canine lumbar segment 2 hemisection spinal cord injury, and the consistency of histological, imageological and behavioral results was compared. AFG was used to construct an aligned fiber bridge that supported cell adhesion in vitro and rapidly facilitated tissue invasion along the long axis of fibers in vivo, Moreover, in vivo results demonstrated regrowth of axons in an oriented pattern connecting the rostral and caudal stumps. Consistent results were confirmed by diffusion tensor imaging, which allowed successful tracing of reconnected nerve fibers across the defect. As a result, directional axonal regrowth contributed to significantly improved recovery of motor functional behavior of SCI canines with AFG implantation. Our results suggest that AFG has great promise for rapidly directing axonal regrowth for nerve regeneration.

中文翻译：

---

对齐的纤维蛋白纳米纤维水凝胶诱导的定向轴突再生长有助于改善犬L2脊髓损伤中的运动功能恢复。

脊髓损伤（SCI）通常会破坏脊髓的长轴突并造成永久性神经功能缺损，目前尚缺乏有效的治疗方法。基于生物材料的再生医学是通过生物材料传递生物物理和/或生化调节信号来诱导轴突再生的关键策略。我们以前制造了一种分层排列的纤维蛋白水凝胶（AFG），该蛋白可促进体外干细胞的神经源性分化，并已成功地应用于大鼠周围神经和脊髓的再生。在这项研究中，AFG用于修复犬的腰段2半切脊髓损伤，并比较了组织学，影像学和行为学结果的一致性。AFG用于构建对齐的纤维桥，该桥在体外支持细胞粘附并在体内迅速促进组织沿纤维长轴的侵袭。此外，体内结果表明轴突以连接头端和尾端残端的定向方式再生。弥散张量成像证实了一致的结果，可以成功追踪整个缺损处重新连接的神经纤维。结果，定向轴突再生长有助于通过AFG植入显着改善SCI犬运动功能行为的恢复。我们的结果表明，AFG对于快速指导轴突再生以促进神经再生具有巨大的希望。体内结果表明轴突再生以连接鼻尖和尾端残端的定向模式。弥散张量成像证实了一致的结果，可以成功追踪整个缺损处重新连接的神经纤维。结果，定向轴突再生长有助于通过AFG植入显着改善SCI犬运动功能行为的恢复。我们的结果表明，AFG对于快速指导轴突再生以促进神经再生具有巨大的希望。体内结果表明轴突再生以连接鼻尖和尾端残端的定向模式。弥散张量成像证实了一致的结果，可以成功追踪整个缺损处重新连接的神经纤维。结果，定向轴突再生长有助于通过AFG植入显着改善SCI犬运动功能行为的恢复。我们的结果表明，AFG对于快速指导轴突再生以促进神经再生具有巨大的希望。定向轴突再生长有助于通过AFG植入显着改善SCI犬的运动功能行为的恢复。我们的结果表明，AFG对于快速指导轴突再生以促进神经再生具有巨大的希望。定向轴突再生长有助于通过AFG植入显着改善SCI犬的运动功能行为的恢复。我们的结果表明，AFG对于快速指导轴突再生以促进神经再生具有巨大的希望。