Objective

The objective of this study is to investigate the effect of IL-18 on intrauterine infection of HBV (Hepatitis B Virus) in mice based on cellular and molecular level, and to analyze its mechanism, as well as the relationship between IL-18 and intrauterine infection of HBV.

Methods

Pregnant rats are taken as the study subjects and divided into two groups according to infection and non-infection, namely the study group and the control group. Firstly, the peripheral blood of rats and the blood of newborn mice are collected for the determination of hepatitis B in two-and-a-half pairs. Then, the levels of interleukin-18 (IL-18), interferon-γ (IFN-γ) and interleukin-4 (IL-4) in peripheral serum are detected by ELISA (Enzyme Linked Immunosorbent Assay). Finally, the two groups of horizontal values are compared and analyzed. The effect of IL-18 on intrauterine infection of HBV in mice is investigated based on the level of cell and molecular.

Results

The levels of IL-18, IFN-γ, IL-4 and IFN-γ/IL-4 in the two groups are compared and analyzed. The levels of IL-18, IFN-γ and IFN-γ/IL-4 in the study group are significantly lower than those in the control group, with statistical significance. However, the level of IL-4 in the study group is higher than that in the control group, with statistical significance.

Conclusion

It is found that the decrease of HL-type specific response and the enhancement of Th2-type specific response in pregnant mice are closely related to HBV intrauterine infection. Moreover, the decrease of IL-18 secretion in peripheral blood may cause intrauterine infection of HBV. This study can make people better realize the mechanism of HBV intrauterine infection, and effectively help clinical prevention and treatment of intrauterine infection.

中文翻译：

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IL-18在细胞分子水平上对小鼠HBV宫内感染的影响。

客观的

本研究旨在从细胞和分子水平探讨IL-18对小鼠HBV（乙型肝炎病毒）宫内感染的影响，并分析其作用机制，以及IL-18与宫内感染的关系。乙肝病毒感染。

方法

以孕鼠为研究对象，按感染和非感染分为两组，即研究组和对照组。首先采集大鼠外周血和新生小鼠血液，分两对半进行乙肝检测。然后，通过ELISA（酶联免疫吸附试验）检测外周血清中白细胞介素18（IL-18）、干扰素-γ（IFN-γ）和白细胞介素4（IL-4）的水平。最后对两组水平值进行比较分析。基于细胞和分子水平研究IL-18对小鼠HBV宫内感染的影响。

结果

比较分析两组IL-18、IFN-γ、IL-4和IFN-γ/IL-4的水平。研究组IL-18、IFN-γ和IFN-γ/IL-4水平显着低于对照组，具有统计学意义。但研究组IL-4水平高于对照组，具有统计学意义。

结论

发现妊娠小鼠HL型特异性反应的降低和Th2型特异性反应的增强与HBV宫内感染密切相关。此外，外周血中IL-18分泌减少可能导致HBV宫内感染。本研究可以使人们更好地认识HBV宫内感染的机制，有效帮助临床防治宫内感染。