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Evaluation of larvicidal efficacy of Ricinus communis (Castor) and synthesized green silver nanoparticles against Aedes aegypti L.
Saudi Journal of Biological Sciences Pub Date : 2020-04-21 , DOI: 10.1016/j.sjbs.2020.04.025
Muhammad Waris 1 , Shabab Nasir 1 , Sadia Abbas 1 , Muhammad Azeem 1 , Bilal Ahmad 1 , Naveed Ahmad Khan 2 , Bilal Hussain 1 , K A Al-Ghanim 3 , F Al-Misned 3 , N Mulahim 3 , Shahid Mahboob 3
Affiliation  

Aedes mosquitoes are the most important group of vectors that transmit pathogens, including arboviruses, and cause human diseases such as dengue fever, yellow fever, Zika virus, and Chikungunya. Biosynthesis and the use of green silver nanoparticles (AgNPs) is a vital step to identify reliable and eco-friendly controls for these vectors. In this study, Aedes (Ae.) aegypti larvae (2nd and 3rd instar) were exposed to leaf extracts of Ricinus communis (Castor) and AgNPs synthesized from the extract to evaluate their larvicidal potential. Synthesized AgNPs were characterized by UV–Vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), and energy-dispersive X-ray spectroscopy (XRD). Ae. aegypti larvae were treated with different concentrations (50–250 ppm) of the leaf extract and synthesized AgNPs. There were five replicates per treatment, in addition to a positive (temephos) and negative control (dechlorinated water). Mortality was recorded after 12, 24, 36, and 48 h and the data were subjected to Probit analysis. The nanoparticles were more toxic (LC50 = 46.22 ppm and LC90 = 85.30 ppm) than the plant extract (106.24 and 175.73 ppm, respectively). The leaf extracts of Ricinus communis were subjected to HPLC analysis to identify their chemical constituents. This study suggests that plant extracts and synthesized nanoparticles are excellent alternatives to hazardous chemical pesticides used to control vector mosquitoes. This is a potentially useful technique that can reduce aquatic toxicity from insecticide use.



中文翻译:

蓖麻和合成绿色银纳米粒子对埃及伊蚊杀幼虫功效的评估。

伊蚊是传播病原体(包括虫媒病毒)并引起登革热、黄热病、寨卡病毒和基孔肯雅热等人类疾病的最重要的媒介群体。绿色银纳米颗粒 (AgNP) 的生物合成和使用是确定这些载体的可靠且环保的控制方法的重要一步。在这项研究中,将埃及伊蚊( Ae .)幼虫(2 龄和 3 龄)暴露于蓖麻(Castor) 叶提取物和从提取物中合成的 AgNP,以评估其杀幼虫潜力。通过紫外-可见光谱、傅里叶变换红外光谱 (FTIR) 和能量色散 X 射线光谱 (XRD) 对合成的 AgNP 进行了表征。艾。用不同浓度(50-250 ppm)的叶提取物处理埃及伊蚊幼虫并合成 AgNP。除了阳性对照(替美磷)和阴性对照(脱氯水)外,每个处理有五个重复。12、24、36和48小时后记录死亡率并对数据进行Probit分析。纳米颗粒的毒性(LC 50  = 46.22 ppm 和 LC 90  = 85.30 ppm)比植物提取物(分别为 106.24 和 175.73 ppm)更高。对蓖麻叶提取物进行 HPLC 分析,鉴定其化学成分。这项研究表明,植物提取物和合成纳米颗粒是用于控制媒介蚊子的危险化学农药的绝佳替代品。这是一种潜在有用的技术,可以减少杀虫剂使用对水生生物的毒性。

更新日期:2020-04-21
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