Pathological angiogenesis characterized by uncontrollable vessel growth is an accompanying feature of many diseases. The avian embryo chorioallantoic membrane (CAM) is an excellent model for angiogenesis research. In our study we used a less common Japanese quail CAM model for the testing of angiogenic potential of leptin, high-molecular (heparin sodium) andlow-molecular (nadroparin calcium) heparins. Heparins play a significant role in vascular endothelial cell function, and they are able to modulate the activities of angiogenic growth factors. On embryonic day 7 leptin (5 μg per CAM), heparin sodium (75 IU per CAM) and nadroparin calcium (47.5 IU per CAM) in 500 μl PBS were applied on the CAM surface. After 24 h the fractal dimension (Df) of the vasculature was evaluated. Samples from each group were histologically analyzed and VEGF-A and Quek1 expression were detected by qPCR. Df was significantly increased in the leptin group. A moderate stimulatory effect of heparin sodium and an inhibitory effect of nadroparin calcium were observed. Both leptin and heparin sodium caused a noticeable increase in the CAM thickness compared to the control and nadroparin calcium groups. We observed an increased number of blood vessels and accumulation of fibroblasts. There was no significant impact on gene expression of VEGF-A and Quek1 24 h after treatment, however, trends similar to the changes in Df and CAM thickness were present. The resulting effect of nadroparin administration on Quek1 levels was exactly the opposite to that of leptin (p < 0.05).

以无法控制的血管生长为特征的病理性血管生成是许多疾病的伴随特征。禽胚绒膜尿囊膜（CAM）是用于血管生成研究的优秀模型。在我们的研究中，我们使用了一种不太常见的日本鹌鹑CAM模型来测试瘦素，高分子（肝素钠）和低分子（萘达林钙）肝素的血管生成潜力。肝素在血管内皮细胞功能中起着重要作用，并且它们能够调节血管生成生长因子的活性。在胚胎的第7天，将瘦素（每个CAM 5μg），肝素钠（每个CAM 75 IU）和萘达泊林钙（每个CAM 47.5 IU）溶于500μlPBS中。24小时后，评估脉管系统的分形维数（Df）。对每组样品进行组织学分析并通过qPCR检测VEGF-A和Quek1表达。瘦素组中Df显着增加。观察到了肝素钠的适度刺激作用和萘达帕林钙的抑制作用。瘦素和肝素钠均导致CAM厚度显着增加（与对照组和萘普林钙相比）。我们观察到血管数量的增加和成纤维细胞的积累。治疗后24小时对VEGF-A和Quek1的基因表达没有显着影响，但是，存在与Df和CAM厚度变化相似的趋势。萘普灵给药对Quek1水平产生的效果与瘦素（p <0.05）。