Toxic metal contamination in soils due industrialization is nowadays a concern to the scientists worldwide. The current study deals with the evaluation of response and tolerance by isolated metallophilic bacteria in different lead concentrations (100 ppm to 1000 ppm). By taking optical densities of the isolates, the minimum inhibitory concentration (MIC) of Pb²⁺ were determined.16S rRNA and MALDI-TOF MS were used for the identification of the bacteria. Total of 37 isolates were observed, among them 04 (Staphylococcus equorum, Staphylococcus warneri, Bacillus safensis and Bacillus thuringiensis), isolated were detected having efficacy of Pb²⁺tolerance and sequestration at varying MIC. Furthermore, B. thuringiensis was observed to have highest (900 ppm) tolerance for lead and lowest (500 ppm) for Staphylococcus warneri. Moreover, the highest (65.3%) sequestration potential has been observed for B. thuringiensis and least (52.8%) for S. warneri. The tolerance and sequestration potential properties of these isolated species can be utilised to exterminate heavy metals and reduce their toxicity from the contaminated environment.

由于工业化，土壤中的有毒金属污染如今已成为全世界科学家关注的问题。当前的研究涉及不同铅浓度（100 ppm至1000 ppm）中分离的嗜金属细菌的响应和耐受性评估。通过对分离物的光密度测定，确定了Pb ²⁺的最小抑菌浓度（MIC）。采用16S rRNA和MALDI-TOF MS鉴定细菌。共观察到37株分离株，其中04株（马氏葡萄球菌，华氏葡萄球菌，安全芽孢杆菌和苏云金芽孢杆菌）分离出，具有不同的MIC对Pb ^2+的耐受性和螯合效果。此外，苏云金芽孢杆菌对铅的耐受性最高（900 ppm），而对金黄色葡萄球菌的耐受性最低（500 ppm）。此外，苏云金芽孢杆菌的隔离潜力最高（65.3 ％），华纳链球菌的隔离潜力最低（52.8％）。这些分离出的物种的耐受性和螯合潜在特性可用于消灭重金属并降低其在污染环境中的毒性。